The Cytotoxicity, DNA Fragmentation, and Decreasing Velocity Induced By Chromium(III) Oxide on Rainbow Trout Spermatozoa

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dc.authoridGürses, Canbolat/0000-0002-4085-0224
dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridOzgur, Mustafa Erkan/0000-0002-2966-9627
dc.authoridKoytepe, Suleyman/0000-0002-4788-278X
dc.authorwosidGürses, Canbolat/AAA-6825-2019
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorOzgur, Mustafa Erkan
dc.contributor.authorUlu, Ahmet
dc.contributor.authorGurses, Canbolat
dc.contributor.authorOzcan, Imren
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorKoytepe, Suleyman
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:51:50Z
dc.date.available2024-08-04T20:51:50Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe present study aimed to determine the cytotoxicity of chromium(III) oxide micro particles (Cr2O3-Ps) in rainbow trout (Oncorhynchus mykiss) spermatozoa. Firstly, Cr2O3-Ps were synthesized and structurally characterized the surface, morphological for particle size and thermal properties. In addition, its structural and elemental purity was determined using energy-dispersive X-ray (EDX) spectrum and elemental maps. Structural purity, thermal properties, and stability of Cr2O3 -Ps were also examined in detail by performing thermal analysis techniques. The cytotoxicity of Cr2O3-Ps was measured by the observation of velocities, antioxidant activities, and DNA damages in rainbow trout spermatozoa after exposure during 3 h in vitro incubation. The straight line velocity (VSL), the curvilinear velocity (VCL), and the angular path velocity (VAP) of spermatozoa decreased after exposure to Cr2O3-Ps. While the superoxide dismutase (SOD) and the catalase (CAT) decreased, the lipid peroxidation increased in a dose-dependent manner. However, the total glutathione (tGSH) was not affected in this period. DNA damages were also determined in spermatozoa using Comet assay. According to DNA in tail (%) data, DNA damages have been detected with gradually increasing concentrations of Cr2O3-Ps. Furthermore, all of class types which are categorized as the intensity of DNA fragmentation has been observed between 50 and 500 mu g/L concentrations of Cr2O3-Ps exposed to rainbow trout spermatozoa. At the end of this study, we determined that the effective concentrations (EC50) were 76.67 mu g/L for VSL and 87.77 mu g/L for VCL. Finally, these results about Cr2O3-Ps may say to be major risk concentrations over 70 mu g/L for fish reproduction in aquatic environments.en_US
dc.description.sponsorshipphysicochemical and biochemical laboratories in Inonu University; Malatya Turgut ozal Universityen_US
dc.description.sponsorshipAnalyses for study were supported by physicochemical and biochemical laboratories in Inonu University and Malatya Turgut ozal University. Therefore, the authors are grateful to the universities for providing the resources to develop this study.en_US
dc.identifier.doi10.1007/s12011-022-03211-9
dc.identifier.endpage983en_US
dc.identifier.issn0163-4984
dc.identifier.issn1559-0720
dc.identifier.issue2en_US
dc.identifier.pmid35368229en_US
dc.identifier.scopus2-s2.0-85127553178en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage968en_US
dc.identifier.urihttps://doi.org/10.1007/s12011-022-03211-9
dc.identifier.urihttps://hdl.handle.net/11616/100589
dc.identifier.volume201en_US
dc.identifier.wosWOS:000777529900002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringernatureen_US
dc.relation.ispartofBiological Trace Element Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCr2O3en_US
dc.subjectCytotoxicityen_US
dc.subjectOncorhynchus mykissen_US
dc.subjectSpermatozoaen_US
dc.subjectDNA damagesen_US
dc.titleThe Cytotoxicity, DNA Fragmentation, and Decreasing Velocity Induced By Chromium(III) Oxide on Rainbow Trout Spermatozoaen_US
dc.typeArticleen_US

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