Insertion- and deletion-associated genetic diversity of Mycobacterium tuberculosis phospholipase C-encoding genes among 106 clinical isolates from Turkey

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dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.contributor.authorTalarico, S
dc.contributor.authorDurmaz, R
dc.contributor.authorYang, ZH
dc.date.accessioned2024-08-04T20:13:45Z
dc.date.available2024-08-04T20:13:45Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBacterial phospholipase C has been reported to play a role in the pathogenesis of many bacteria. In order to gain a better understanding of the potential role of Mycobacterium tuberculosis phospholipase C in the pathogenesis of human tuberculosis, we investigated the genetic diversity of the four M. tuberculosis phospholipase Gencoding genes (plcA, plcB, plcC, and plcD) resulting from the IS6110 insertion and associated deletion, among 106 clinical isolates obtained from Turkey, by using PCR, Southern hybridization, and DNA sequencing. Two sequenced M. tuberculosis strains, H37Rv and CDC1551, were used as the references in the comparison. Sixty-six (62.3%) of the 106 isolates had an intact plcD gene, and 40 (37.7%) showed an interruption of the gene. Of the latter 40 isolates, 19 (47.5%) had an IS6110 insertion with no associated deletion in the plcD gene, 2 (5%) had an IS6110 insertion and an associated deletion within the plcD gene, 15 (37.5%) had an IS6110 insertion in the plcD gene that was associated with a partial deletion of the plcD gene and its right forward adjacent region, and 4 (10%) had a complete deletion of the plcD gene. The proportions of the isolates with an interrupted plcA, plcB, or plcC gene were 3.8, 1.9, and 3.8%, respectively. The data indicate that there is a much higher frequency of IS6110 insertion and deletion in the plcD gene than in the plcA, plcB, and plcC genes of M. tuberculosis.en_US
dc.description.sponsorshipNIAID NIH HHS [R01-AI51975] Funding Source: Medlineen_US
dc.identifier.doi10.1128/JCM.43.2.533-538.2005
dc.identifier.endpage538en_US
dc.identifier.issn0095-1137
dc.identifier.issn1098-660X
dc.identifier.issue2en_US
dc.identifier.pmid15695641en_US
dc.identifier.scopus2-s2.0-13844256145en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage533en_US
dc.identifier.urihttps://doi.org/10.1128/JCM.43.2.533-538.2005
dc.identifier.urihttps://hdl.handle.net/11616/93810
dc.identifier.volume43en_US
dc.identifier.wosWOS:000227045600001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Soc Microbiologyen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectListeria-Monocytogenesen_US
dc.subjectPseudomonas-Aeruginosaen_US
dc.subjectVirulenceen_US
dc.subjectSequenceen_US
dc.subjectRolesen_US
dc.titleInsertion- and deletion-associated genetic diversity of Mycobacterium tuberculosis phospholipase C-encoding genes among 106 clinical isolates from Turkeyen_US
dc.typeArticleen_US

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