Investigation of clonal relationship in Klebsiella pneumoniae strains grown in invasive specimens obtained from intensive care units

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dc.contributor.authorGuducuoglu, Huseyin
dc.contributor.authorDavarci, Ismail
dc.contributor.authorTanriverdi, Elif Seren
dc.contributor.authorArabaci, Furkan
dc.contributor.authorKirilmaz, Eda
dc.contributor.authorAldi, Feza Irem
dc.contributor.authorOtlu, Baris
dc.date.accessioned2026-04-04T13:30:52Z
dc.date.available2026-04-04T13:30:52Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractIntroduction: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a bacterium in critical category on the World Health Organization's list of priority pathogens. The aim of our study is to identify the carbapenem resistance genes of K. pneumoniae isolates obtained from blood and cerebrospinal fluid samples sent from the intensive care units of our hospital and to investigate the clonal relationship among them. Methodology: K. pneumoniae strains isolated from blood and CSF samples routinely collected from the intensive care units of our hospital over a two-year period were included in the study. Carbapenemase screening of CRKP strains was performed using Carba NP and CIM tests. Additionally, carbapenemase resistance genes (blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48) and clone analysis were performed using AP-PCR on these strains. Results: A total of 186 strains with reduced susceptibility to at least one carbapenem were detected. In carbapenemase screening, the sensitivity of the CIM test was 98.3% (169/172), specificity was 7.1% (1/14); the sensitivity of Carba NP was 90.7% (156/172), specificity was 78.6% (11/14). 83.9% of the strains were blaOXA-48, 4.8% blaNDM, and 3.8% blaKPC positive. blaIMP and blaVIM resistance genes were not detected. One hundred and eighty-six K. pneumoniae isolates were identified with 62 different genotypes, and isolates showing clustering were grouped into 30 different clusters. The clustering rate of these isolates was 82.8%. Conclusions: Resistant bacteria can cause small outbreaks in ICUs. Therefore, to identify high-risk clones and prevent further spread, there is a need to increase capacity to support outbreak investigations and surveillance with real-time whole genome sequencing.
dc.description.sponsorshipTrakya University Scientific Projects [2023-141]
dc.description.sponsorshipAcknowledgements This Project was supported by Project No: 2023-141 within the scope of Trakya University Scientific Projects. We would also like to thank the Trakya University Medical Microbiology staff.
dc.identifier.doi10.3855/jidc.21195
dc.identifier.issn1972-2680
dc.identifier.issue11
dc.identifier.orcid0000-0002-8563-2386
dc.identifier.orcid0000-0002-0449-0356
dc.identifier.orcid0000-0002-3713-8930
dc.identifier.orcid0000-0002-6220-0521
dc.identifier.pmid41358760
dc.identifier.scopus2-s2.0-105024146579
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.3855/jidc.21195
dc.identifier.urihttps://hdl.handle.net/11616/108443
dc.identifier.volume19
dc.identifier.wosWOS:001650746800001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherJ Infection Developing Countries
dc.relation.ispartofJournal of Infection in Developing Countries
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectbloodstream infection
dc.subjectcarbapenem-resistant
dc.subjectclonal relationship
dc.subjectKlebsiella pneumoniae
dc.subjectmultidrug-resistant
dc.titleInvestigation of clonal relationship in Klebsiella pneumoniae strains grown in invasive specimens obtained from intensive care units
dc.typeArticle

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