Sisplatin ile indüklenen sıçanlarda testiküler toksisiteye karşı astaksantinin koruyucu etkileri
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Sisplatinin testis üzerindeki hasarına karşı astaksantinin biyokimyasal, histopatolojik ve immünohistokimyasal yöntemlerle değerlendirip sonuçlandırmayı amaçladık. Yöntem ve Gereç: 36 adet Wistar Albino cinsi erkek sıçanlar kullanıldı ve 4 gruba ayrılarak kontrol ve astaksantin grubunda 8, sisplatin ve sisplatin+astaksantin grubunda 10 adet sıçan olacak şekilde ayarlandı. Deney sonunda dokular toplandı ve histolojik analizler için H-E ve immünohistokimya için Nrf2, HO-1, Vimentin, Kaspaz-3, PCNA ve 3?-HSD ile boyandı. Biyokimyasal analizler için kalpten alınan kanlardan testosteron hormon ve testis dokularından MDA ve tGSH analizi yapıldı. Bulgular: Biyokimyasal sonuçlara göre sisplatin grubunda testosteron seviyesinde ve tGSH düzeyinde anlamlı bir azalma, MDA düzeyinde ise anlamlı bir artış gözlendi. İmmünohistokimyasal sonuçlara göre Nrf2, HO-1, vimentin ve kaspaz-3 immünreaktivitesinde artış, 3?-HSD ise anlamlı bir azalma gözlendi. Histopatolojik sonuçlara göre seminifer epitel ve tübül çapları sisplatin grubunda anlamlı bir azalma gözlendi. Deney öncesi ve sonrası vücut ağırlıkları sisplatin grubunda anlamlı bir azalma, testis ağırlıkları karşılaştırıldığında ise belirgin bir azalma olmasına rağmen istatistiksel olarak anlamlı değildi. Sonuç: Sisplatinin testis üzerindeki sitotoksik etkisine karşı astaksantinin koruyucu ve tedavi edici etkinliği, çalışmamızın sonuçları ile kanıtlanmıştır. Anahtar Kelimeler: Astaksantin, Apoptoz, İnflamasyon, Testis hasarı, Sisplatin, Oksidatif stress.
Aim: We aimed to evaluate and conclude the effects of astaxanthin against cisplatin-induced testicular damage using biochemical, histopathological, and immunohistochemical methods. Material and Methods: Thirty-six male Wistar Albino rats were used and divided into 4 groups: control and astaxanthin groups with 8 rats each, and cisplatin and cisplatin+astaxanthin groups with 10 rats each. At the end of the experiment, tissues were collected and stained with H-E for histological analyses and with Nrf2, HO-1, Vimentin, Caspase-3, PCNA, and 3?-HSD for immunohistochemistry. For biochemical analyses, testosterone hormone levels were measured from blood taken from the heart, and MDA and tGSH levels were measured from testicular tissues. Results: According to the biochemical results, a significant decrease in testosterone levels and tGSH levels and a significant increase in MDA levels were observed in the cisplatin group. According to the immunohistochemical results, an increase in the immunoreactivity of Nrf2, HO-1, vimentin, and caspase-3 and a significant decrease in 3?-HSD were observed. According to the histopathological results, a significant decrease in seminiferous epithelial and tubule diameters was observed in the cisplatin group. Pre- and post-experiment body weights showed a significant decrease in the cisplatin group, whereas testis weights showed a marked decrease, but it was not statistically significant. Conclusion: The protective and therapeutic efficacy of astaxanthin against the cytotoxic effect of cisplatin on the testes has been demonstrated by the results of our study. Keywords: Astaxanthin, Apoptosis, Cisplatin, İnflammation, Testicular damage, Oxidative stress.
Aim: We aimed to evaluate and conclude the effects of astaxanthin against cisplatin-induced testicular damage using biochemical, histopathological, and immunohistochemical methods. Material and Methods: Thirty-six male Wistar Albino rats were used and divided into 4 groups: control and astaxanthin groups with 8 rats each, and cisplatin and cisplatin+astaxanthin groups with 10 rats each. At the end of the experiment, tissues were collected and stained with H-E for histological analyses and with Nrf2, HO-1, Vimentin, Caspase-3, PCNA, and 3?-HSD for immunohistochemistry. For biochemical analyses, testosterone hormone levels were measured from blood taken from the heart, and MDA and tGSH levels were measured from testicular tissues. Results: According to the biochemical results, a significant decrease in testosterone levels and tGSH levels and a significant increase in MDA levels were observed in the cisplatin group. According to the immunohistochemical results, an increase in the immunoreactivity of Nrf2, HO-1, vimentin, and caspase-3 and a significant decrease in 3?-HSD were observed. According to the histopathological results, a significant decrease in seminiferous epithelial and tubule diameters was observed in the cisplatin group. Pre- and post-experiment body weights showed a significant decrease in the cisplatin group, whereas testis weights showed a marked decrease, but it was not statistically significant. Conclusion: The protective and therapeutic efficacy of astaxanthin against the cytotoxic effect of cisplatin on the testes has been demonstrated by the results of our study. Keywords: Astaxanthin, Apoptosis, Cisplatin, İnflammation, Testicular damage, Oxidative stress.
Açıklama
Anahtar Kelimeler
Histoloji ve Embriyoloji, Histology and Embryology
