Sıçanlarda oluşturulan deneysel serebral iskemi/reperfüzyon hasarı üzerine intranazal Salusin-ß uygulamasının etkilerinin araştırılması
Küçük Resim Yok
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: İntranazal Salusin-? uygulamasının serebral iskemi/reperfüzyon (Sİ/R) hasarındaki muhtemel koruyucu etkilerinin araştırılması amaçlandı. Materyal ve metot: Çalışmada 27 adet Sprague Dawley ırkı erkek sıçan kontrol, Sİ/R ve Sİ/R+ Salusin-? olmak üzere üç gruba (n=9) ayrıldı. Kontrol dışındaki gruplara 90 dakikalık serebral iskemi (Sİ) ve sonrasında 3 gün reperfüzyon uygulandı. Sİ'den bir saat sonra intraperitoneal yolla Sİ/R grubuna hidroksietil selüloz, Sİ/R+Salusin-? grubuna ise intranazal olarak 300nM Salusin-? infüzyonu uygulandı. Üç günlük reperfüzyon boyunca sıçanlara nörolojik defisit skorlama (NDS), rotarod, yapışkan çıkarma ve kavrama gücü testleri yaptırıldı. Deney sonunda hayvanlar dekapite edilerek beyin dokuları alındı. Alınan beyin dokularında TTC boyama yöntemi ile infarkt alan, Western Blot yöntemi ile apoptoz (BCL-2) ve otofaji (Beclin-1, ATG5, ATG7 ve p62) proteinlerinin seviyeleri belirlendi. Bulgular: Kontrol ve Sİ/R+Salusin-? gruplarının NDS skoru ve yapışkan çıkarma süresi, Sİ/R grubuna göre düşük; rotarodda kalma süresi ve kavrama gücü yüksekti (p<0.05). İnfarkt alanının Sİ/R grubunda, kontrol ve Sİ/R+Salusin-? gruplarına kıyasla büyük olduğu belirlendi (p<0.05). BCL-2, Beclin-1, ATG5 ve ATG7 protein seviyelerinin, Sİ/R+ Salusin-? grubunda Sİ/R grubuna göre yüksek ve p62 protein seviyelerinin azaldığı tespit edildi (p<0.05). Sonuç: Salusin-?' nın NDS'yi düşürdüğü görüldü. Davranış testlerinde de denge-motor koordinasyonu, somatosensör ve güç kaybını iyileştirdiği saptandı. Bunların yanı sıra apoptotik protein seviyelerinde apoptozu inhibe edecek yönde otofajik protein seviyelerinde otofajiyi indükleyecek yönde etkilediği tespit edildi. Anahtar Kelimeler: Apoptoz, Salusin-?, Serebral İskemi, Otofaji
Aim: The aim was to investigate the potential protective effects of intranasal Salusin-? administration in cerebral ischemia/reperfusion(I/R) injury. Material and method: The Sprague Dawley male rats included in the study were divided into three groups (n=9): control, I/R, and I/R+Salusin-?. In the groups other than the control, a 90-minute ischemia was applied. One hour after ischemia, the I/R group received intraperitoneal hydroxyethyl cellulose, while the I/R+Salusin-? group was administered 300 nM Salusin-? intranasally. During the three-day reperfusion period, the rats underwent NDS, rotarod, sticky label removal, and grip strength tests. At the end of experiment animals were decapitated and brain tissues were collected. In collected brain tissues, infarct area was determined using TTC staining, and the levels of apoptosis (BCL-2) and autophagy (Beclin-1, ATG5, ATG7, and p62) proteins were analyzed using Western Blot method. Results: The NDS score and sticky label removal time of the control and I/R+Salusin-? groups were lower, while the rotarod endurance time and grip strength were higher compared to the I/R group (p<0.05). The infarct area in the I/R group was found to be larger compared to the control and I/R+Salusin-? groups(p<0.05). The levels of BCL-2, Beclin-1, ATG5, and ATG7 proteins were higher in the I/R+Salusin-? group compared to I/R group, while the levels of p62 proteins were lower(p<0.05). Conclusion: It was observed that Salusin-? reduced neurological deficit score. Behavioral tests showed improvement in balance-motor coordination, somatosensory functions, and strength loss. Additionally, it was found that Salusin-? reduced the infarct area caused by I/R injury. Furthermore, Salusin-? was found to inhibit apoptosis by affecting apoptotic protein levels and induce autophagy by influencing autophagic protein levels. Keywords: Apoptoz, Salusin-?, Cerebral Ischemia, Autophagy
Aim: The aim was to investigate the potential protective effects of intranasal Salusin-? administration in cerebral ischemia/reperfusion(I/R) injury. Material and method: The Sprague Dawley male rats included in the study were divided into three groups (n=9): control, I/R, and I/R+Salusin-?. In the groups other than the control, a 90-minute ischemia was applied. One hour after ischemia, the I/R group received intraperitoneal hydroxyethyl cellulose, while the I/R+Salusin-? group was administered 300 nM Salusin-? intranasally. During the three-day reperfusion period, the rats underwent NDS, rotarod, sticky label removal, and grip strength tests. At the end of experiment animals were decapitated and brain tissues were collected. In collected brain tissues, infarct area was determined using TTC staining, and the levels of apoptosis (BCL-2) and autophagy (Beclin-1, ATG5, ATG7, and p62) proteins were analyzed using Western Blot method. Results: The NDS score and sticky label removal time of the control and I/R+Salusin-? groups were lower, while the rotarod endurance time and grip strength were higher compared to the I/R group (p<0.05). The infarct area in the I/R group was found to be larger compared to the control and I/R+Salusin-? groups(p<0.05). The levels of BCL-2, Beclin-1, ATG5, and ATG7 proteins were higher in the I/R+Salusin-? group compared to I/R group, while the levels of p62 proteins were lower(p<0.05). Conclusion: It was observed that Salusin-? reduced neurological deficit score. Behavioral tests showed improvement in balance-motor coordination, somatosensory functions, and strength loss. Additionally, it was found that Salusin-? reduced the infarct area caused by I/R injury. Furthermore, Salusin-? was found to inhibit apoptosis by affecting apoptotic protein levels and induce autophagy by influencing autophagic protein levels. Keywords: Apoptoz, Salusin-?, Cerebral Ischemia, Autophagy
Açıklama
Anahtar Kelimeler
Fizyoloji, Physiology
