The effect of carbon dioxide pneumoperitoneum on free radicals

Basit Öğe Kaydı
dc.authoridAsma, Dilek/0000-0002-3866-3016
dc.authoridYesilada, Ozfer/0000-0003-0038-6575
dc.authoridOzmen, Murat/0000-0003-4378-0839
dc.authorwosidOZMEN, MURAT/A-4874-2009
dc.authorwosidAsma, Dilek/AAA-5294-2021
dc.authorwosidYesilada, Ozfer/ABI-1335-2020
dc.contributor.authorSare, M
dc.contributor.authorYilmaz, I
dc.contributor.authorHamamci, D
dc.contributor.authorBirincioglu, M
dc.contributor.authorÖzmen, M
dc.contributor.authorYesilada, Ö
dc.date.accessioned2024-08-04T20:12:08Z
dc.date.available2024-08-04T20:12:08Z
dc.date.issued2000
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Carbon dioxide is usually preferred as the insufflating agent for laparoscopic surgery because it is readily available, noncombustible, and chemically stable. It is still questionable, however, if CO2 pneumoperitoneum has any effect on free radicals and lipid peroxidation. The purpose of this study was to investigate the possible effects of CO2 pneumoperitoneum on free radicals and lipid peroxidation in the erythrocytes of rats. Methods: Fifty male Sprague-Dawley rats were divided into five equal,groups: controls, a sham-operation group, and three groups of 5, 10, or 15 mmHg pneumoperitoneum with CO2. At the end of the procedure, blood was collected and the erythrocytes were separated from the plasma. The resultant supernatant fractions of erythrocytes were assayed For superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA). Results: SOD activities of the 5 and 10 mmHg pneumoperitoneum groups were significantly lower than those of the sham operation group. SOD activity was greater in the 15 mmHg pneumoperitoneum group than in any of the other groups, and this activity was significantly different from that seen in the 5 and 10 mmHg pneumoperitoneum groups (p < 0.05). No significant changes were observed in the CAT activities of the study,groups (p > 0.05). MDA level was increased in the 5 mmHg pneumoperitoneum group; this result was statistically different from the control and 15 mmHg pneumoperitoneum groups (p < 0.05). No significant differences were found in the CAT activities for the study groups. On the other hand, the SOD activities of the 5 and 10 mmHg pneumoperitoneum groups were significantly lower than those of the sham and the 15 mmHg pneumoperitoneum group (p < 0.05 for all comparisons). Conclusions: These results indicate that CO2 pneumoperitoneum applied with 5-10 mmHg pressure increases the formation of free oxygen radicals by inhibiting SOD activity and that the accumulation of free radicals elevates the level of MDA, a metabolite of Lipid peroxidation. The effect of CO2 pneumoperitoneum on free radicals and lipid peroxidation is pressure-dependent in rats. The mechanism underlying this pressure dependency is still under investigation.en_US
dc.identifier.doi10.1007/s004640000168
dc.identifier.endpage652en_US
dc.identifier.issn0930-2794
dc.identifier.issn1432-2218
dc.identifier.issue7en_US
dc.identifier.pmid10948302en_US
dc.identifier.scopus2-s2.0-0033869288en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage649en_US
dc.identifier.urihttps://doi.org/10.1007/s004640000168
dc.identifier.urihttps://hdl.handle.net/11616/93235
dc.identifier.volume14en_US
dc.identifier.wosWOS:000088516200010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofSurgical Endoscopy and Other Interventional Techniquesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcarbon dioxideen_US
dc.subjectcatalaseen_US
dc.subjectlaparoscopic surgeryen_US
dc.subjectmalondialdehydeen_US
dc.subjectsuperoxide dismutaseen_US
dc.titleThe effect of carbon dioxide pneumoperitoneum on free radicalsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu