Development and validation of a RP-HPLC method for determination of solubility of furosemide

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dc.authorscopusid14032701900
dc.authorscopusid55768801700
dc.contributor.authorKaynak M.S.
dc.contributor.authorSaffln S.
dc.date.accessioned2024-08-04T20:02:10Z
dc.date.available2024-08-04T20:02:10Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe objective of the Biopharmaceutics Classification System is to allow prediction of in vivo pharmacokinetic performance of drug products from in vitro measurements; therefore, it is important to determine the solubility and permeability of drug substances. Furosemide (FSM) is a loop diuretic commonly used in the treatment of edematous states associated chronic renal failure, hypertension, congestive heart failure and cirrhosis of the liver. The aim of this study was to develop and validate an HPLC method for quantification of FSM in the samples obtained from the in vitro solubility studies performed at five different pH values (pH 1.0, 2.9, 3.9, 4.9 and 7.5). Chromatographic separation of FSM was achieved on a reverse phase column (Waters Spherisorb ODS2 C18 250x4.6 mm 5 pim) with a mobile phase consisted of 0.01M KH2P04 (pH 5.5) and methanol (70:30 v/v). Analyses were run at a flow rate 1 mL/min and UV detection was performed at 235 nm. Under these conditions, the retention time of FSM was about 7.0 min. The method was linear in the concentration range of 0.5 to 50 ?/mL, and limit of quantification was 320 ng/mL. Developed and validated HPLC method was proved to be simple, reliable and also suitable as a single method for studying the solubility of FSM as a function of pH. Finally, based on our results, solubility of FSM was dependent on pH. Its solubility was low between pH 1.0 and 4.9, and was high at pH 7.5.en_US
dc.identifier.endpage34en_US
dc.identifier.issn1304-530X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84879171553en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage25en_US
dc.identifier.urihttps://hdl.handle.net/11616/91462
dc.identifier.volume10en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBCSen_US
dc.subjectDose numberen_US
dc.subjectFurosemideen_US
dc.subjectHPLC methoden_US
dc.subjectSolubility classen_US
dc.titleDevelopment and validation of a RP-HPLC method for determination of solubility of furosemideen_US
dc.title.alternativeFurosemidin çözünürlü?ünün tayini için Bir RP-HPLC metodun geliştirilmesive validasyonuen_US
dc.typeArticleen_US

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