Designing of rapid assay for the detection of RdRp/Orf1ab specific to SARS-CoV-2

dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridGULTEKIN, Enes/0000-0002-8545-1868
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorDerin, Dilek Cam
dc.contributor.authorGultekin, Enes
dc.contributor.authorTaskin, Irmak Icen
dc.contributor.authorOtlu, Baris
dc.contributor.authorOktem, Huseyin Avni
dc.date.accessioned2024-08-04T20:54:32Z
dc.date.available2024-08-04T20:54:32Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractSARS-CoV-2 is still threat and mostly used detection method is real time reverse transcriptase polymerase chain reaction (rRT-PCR) for the open reading frame (Orf1ab), RNA-dependent RNA polymerase (RdRp), nucleocapsid (N) and envelope (E) genes of virus. However, rRT-PCR may have false negative rate for the nucleic acid detection. Since the RdRp/Orf1ab has high sensitivity for the molecular detection, two sandwich models, Model 1A-Model 1B, based on hybridization on lateral flow assay (LFA) were designed here and applied with the synthetic and clinical samples of RdRp/Orf1ab. In this purpose colloidal gold nanoparticles (AuNPs) were used as label. Membranes having different flow rate, three oligonucleotide probe concentrations and running buffers were used. Although synthetic target sequence was recognized by all the LFAs, PCR products obtained from either the synthetic plasmid DNA or oro/nasopharyngeal swabs were detected by Model 1 A using W12 mem-brane. Designed strip assays detected the RdRp/Orf1ab of the clinical samples as 100% sensitivity and specifity. It means that they might be used for the detection of virus and can be modified for the recognition of mutant genes of virus. These findings also demonstrated the importance of membranes, sandwich models, probe con-centrations and sample contents for developing LFAs for viral detection.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUEBITAK); [120Z962]en_US
dc.description.sponsorshipThis work was supported by Scientific and Technological Research Council of Turkey (TUEBITAK; 120Z962) .en_US
dc.identifier.doi10.1016/j.jviromet.2023.114774
dc.identifier.issn0166-0934
dc.identifier.issn1879-0984
dc.identifier.pmid37460042en_US
dc.identifier.scopus2-s2.0-85165610918en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1016/j.jviromet.2023.114774
dc.identifier.urihttps://hdl.handle.net/11616/101484
dc.identifier.volume320en_US
dc.identifier.wosWOS:001057535000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Virological Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRapid assayen_US
dc.subjectSARS-CoV-2en_US
dc.subjectSandwich assayen_US
dc.subjectRdRp/Orf1aben_US
dc.subjectViral detectionen_US
dc.titleDesigning of rapid assay for the detection of RdRp/Orf1ab specific to SARS-CoV-2en_US
dc.typeArticleen_US

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