Hepatoprotective effects of N-acetylcysteine on liver injury by irisin upregulation and oxidative stress reduction in diabetic rats

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dc.authoridTURK, AHMET/0000-0003-0903-3522
dc.authorwosidTURK, AHMET/HJP-4029-2023
dc.contributor.authorErdogan, Mehmet Mustafa
dc.contributor.authorErdogan, Mehmet Ali
dc.contributor.authorKoc, Suleyman
dc.contributor.authorYalcin, Alper
dc.contributor.authorTurk, Ahmet
dc.contributor.authorYetkin, Esra Akkus
dc.date.accessioned2024-08-04T20:54:30Z
dc.date.available2024-08-04T20:54:30Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackgroundThe current study aimed to investigate the oxidative stress in rat liver with diabetes mellitus (DM) as well as the protective effects of N-acetylcysteine (NAC) on irisin expression.MethodsTwenty-eight male Wistar rats were divided into four groups, 7 rats in each group, and 30-day regimens of experimental or control groups. NAC-treated group is as follows: 100 mg/kg once daily was administered intraperitoneally (i.p.). Diabetes-induced group is as follows: single-dose intraperitoneal injection of streptozotocin (STZ) (50 mg/kg) was used to induce DM in overnight fasting Wistar rats. By determining blood glucose concentration in STZ-induced rats 72 h after injection of STZ, DM was assessed. DM + NAC group is as follows: STZ-induced DM plus NAC is described previously. On the 30th day of the experiment, liver samples were collected after fasting and anesthesia. Biochemical analyses were performed to measure total antioxidant status (TAS), total oxidant status (TOS), and malondialdehyde (MDA) levels. Each liver sample was weighed and then prepared for histopathologic evaluation by light microscopy.ResultsThere was a statistically significant decrease in TAS levels and an increase in TOS and MDA levels in the DM group compared to the control group. In contrast, TOS and MDA levels were found significantly decreased, and TAS levels increased in the serum and liver tissues of the DM + NAC group compared to the DM group. Liver samples were also used for histopathological examination using hematoxylin-eosin and immunohistochemical staining. STZ-induced liver damage was detected as oxidative stress, increased irisin immunoreactivity, sinusoidal dilatation, and hepatocyte degeneration. In the DM + NAC group, it was observed that NAC significantly reduced the aforementioned histopathological changes due to STZ.ConclusionIn the early period of diabetes, due to the antioxidant properties of irisin related to the sudden response of liver tissue to oxidative stress, it is thought that the immunoreactivity in the tissue increases in the early period. As a result, NAC in diabetic rat liver tissue was found to suppress oxidative damage and irisin immunoreactivity.en_US
dc.identifier.doi10.1186/s43066-023-00271-x
dc.identifier.issn2090-6218
dc.identifier.issn2090-6226
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85164338591en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.1186/s43066-023-00271-x
dc.identifier.urihttps://hdl.handle.net/11616/101459
dc.identifier.volume13en_US
dc.identifier.wosWOS:001025840900002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeropenen_US
dc.relation.ispartofEgyptian Liver Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIrisinen_US
dc.subjectLiver toxicityen_US
dc.subjectN-acetylcysteineen_US
dc.subjectOxidative stressen_US
dc.subjectDiabetesen_US
dc.titleHepatoprotective effects of N-acetylcysteine on liver injury by irisin upregulation and oxidative stress reduction in diabetic ratsen_US
dc.typeArticleen_US

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