Simultaneous determination of acyclovir, metoprolol and phenol red by a RP-HPLC method for intestinal perfusion studies

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dc.authorscopusid56960016500
dc.authorscopusid14032701900
dc.authorscopusid57221923900
dc.contributor.authorAteş M.
dc.contributor.authorKaynak M.S.
dc.contributor.authorŞahin S.
dc.date.accessioned2024-08-04T20:00:59Z
dc.date.available2024-08-04T20:00:59Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIntestinal perfusion (SPIP) technique is one of the most commonly used techniques to determine the intestinal permeability of a drug. In perfusion studies, metoprolol (as tartrate) and phenol red are widely used as a refer- ence compounds to evaluate the permeability coefficient of the compound of interest (acyclovir in this study). The aim of our study was to develop and validate a reversed-phase liquid chromatographic method for the simultan- eous determination of acyclovir, metoprolol and phenol red for use in intes- tinal perfusion studies. The analysis was performed on a C18 column (4.6 mm x 250 mm, 5 ?m) using a mobile phase consisting of methanol:0.0125 M potassium dihydrogen phosphate buffer (55:45, v/v; pH 7.0). Method was validated according to the FDA guidelines for selectivity, sensitivity, linearity, precision, accuracy, stability. All calibration curves were linear (r2> 0.999). Lower limit of quantitation was 0.04 ?g/mL for acyclovir, 0.02 ?g/mL for metoprolol, 0.01 ?g/mL for phenol red. Detection limit was 0.01 ?g/mL for acyclovir 0.002 ?g/mL for metoprolol, 0.003 ?g/mL for phenol red. Precision and accuracy results of the method fulfilled the required limits. This newly developed and validated method can be readily used on a routine basis for the standardization of in situ intestinal permeability experiments. © 2015, Hacettepe University, Faculty of Pharmacy. All rights reserved.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK: TÜBİTAK-SBAG-111S335; Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKen_US
dc.description.sponsorshipThis work was supported by a grant from The Scientific and Technological Research Council of Turkey (TÜBİTAK-SBAG-111S335)en_US
dc.identifier.endpage161en_US
dc.identifier.issn1300-0608
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84971307677en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage146en_US
dc.identifier.urihttps://hdl.handle.net/11616/91151
dc.identifier.volume35en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherHacettepe University, Faculty of Pharmacyen_US
dc.relation.ispartofHacettepe University Journal of the Faculty of Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcycloviren_US
dc.subjectHPLCen_US
dc.subjectIn situ intestinal perfusionen_US
dc.subjectMetoprololen_US
dc.subjectPhenol reden_US
dc.titleSimultaneous determination of acyclovir, metoprolol and phenol red by a RP-HPLC method for intestinal perfusion studiesen_US
dc.typeArticleen_US

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