ISOLATION AND PURIFICATION OF CUCURBITACIN D AND I FROM ECBALLIUM ELATERIUM (L.) A. RICH FRUIT JUICE

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dc.authoridBaysar, Ahmet/0000-0002-7017-399X
dc.authorwosidTosun, Emir/S-5125-2018
dc.authorwosidBaysar, Ahmet/Q-1190-2018
dc.contributor.authorTosun, Emir
dc.contributor.authorBaysar, Ahmet
dc.date.accessioned2024-08-04T20:47:07Z
dc.date.available2024-08-04T20:47:07Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe objective of this study was to develop a rapid, economic, and efficient method for simultaneous selective isolation, separation, and purification of cucurbitacin D and I from Ecballium elaterium (L.) A. Rich fruit juice via reversed-phase flash chromatography combined with HPLC. The chloroform extract of the fruit juice was fractionated with flash chromatography using a chloroform, acetone and methanol solvent combination at a 5 ml/min flow rate. Then, a validated HPLC method was utilized for purification of the two targeted cucurbitacins. Cucurbitacin D and I were collected automatically by the fraction collector. The fractions containing the same compounds were pooled and lyophilized. The purified cucurbitacin D and I compounds were identified by NMR, LC-MS, and UV spectra analysis. The results suggest that the applied procedure is simple, quick, and highly efficient. The HPLC method was found to be linear, accurate, precise and rugged for the quantification of the cucurbitacins studied.en_US
dc.description.sponsorshipInonu University Scientific and Research Projects Unit [2012/37]en_US
dc.description.sponsorshipThe work was partially supported by Inonu University Scientific and Research Projects Unit (Project No: 2012/37).en_US
dc.identifier.doi10.20450/mjcce.2019.1648
dc.identifier.endpage182en_US
dc.identifier.issn1857-5552
dc.identifier.issn1857-5625
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85079421818en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage171en_US
dc.identifier.urihttps://doi.org/10.20450/mjcce.2019.1648
dc.identifier.urihttps://hdl.handle.net/11616/99181
dc.identifier.volume38en_US
dc.identifier.wosWOS:000508000900003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSoc Chemists Technologists Madeconiaen_US
dc.relation.ispartofMacedonian Journal of Chemistry and Chemical Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcucurbitacin Den_US
dc.subjectcucurbitacin Ien_US
dc.subjectEcballium elaterium (L.) A. Richen_US
dc.subjectisolationen_US
dc.subjectmethod validationen_US
dc.titleISOLATION AND PURIFICATION OF CUCURBITACIN D AND I FROM ECBALLIUM ELATERIUM (L.) A. RICH FRUIT JUICEen_US
dc.typeArticleen_US

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