Carriage of antimicrobial resistance genes in Escherichia coli of bovine origin

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dc.contributor.authorOzbey, G.
dc.contributor.authorTanriverdi, E. S.
dc.contributor.authorAcik, M. N.
dc.contributor.authorKalin, R.
dc.contributor.authorOtlu, B.
dc.contributor.authorZigo, F.
dc.date.accessioned2026-04-04T13:31:25Z
dc.date.available2026-04-04T13:31:25Z
dc.date.issued2024
dc.departmentİnönü Üniversitesi
dc.description.abstractThe present study aimed to search for the presence of the plasmid-mediated antimicrobial resistance genes in 106 Escherichia coli (E. coli) isolates from a total of 240 fresh fecal samples collected from 12 private cattle farms in Bingol province of East Turkey from November 2021 to January 2022. In those colistin-resistant E. coli (mcr-1 to-9), the major carbapenemase ( bla OXA-48 , bla NDM-1 , bla IMP, bla VIM, and bla KPC ), beta-lactamase (blaTEM-1, bla CTX-M and bla SHV-1 ) and OXA-48 like beta- lactamase ( bla OXA-162 , bla OXA-163 , bla OXA-181 , bla OXA-204 and bla OXA-232 ) resistance genes were searched for determined a multiplex polymerase chain reaction (PCR) method and Next-generation sequencing (NGS)- PCR Amplicons with Nanopore Technology. Only the mcr-4 gene was found in one isolate and the remaining genes (mcr-1-9) were not shown in all E. coli isolates from cattle. The minimal inhibitory concentration (MIC) to colistin was detected in mcr-4 positive E. coli isolates using broth microdilution. We assessed the antimicrobial susceptibilities of mcr-4 positive E. coli isolates using the Kirby-Bauer disk diffusion method. E coli isolate was detected as negative for carbapenemase and OXA-48 like beta-lactamase resistance genes and positive for beta-lactamase. In addition, E. coli isolates carrying mcr-4 were more resistant to colistin. Antimicrobial susceptibility testing using the disk diffusion assay indicated that all 106 E. coli isolates (100%) were sensitive to AMK, 105 E. coli isolates (99.1%) exhibited sensitivity to imipenem, meropenem and doripenem, and 1 E. coli isolate (0.9%) had intermediate resistance to imipenem, meropenem and doripenem; It was observed that all strains (100%) were resistant to cefotaxime. E. coli isolates are resistant to ampicillin (95.3%), amoxicillin/clavulanic acid (95.3%), cefepime (14.2%), cefixime (19.8%), cephalexin (74.5%), gentamicin (42.5%), kanamycin (37.7%), streptomycin (69.8%), tetracycline (80.2%), ciprofloxacin (60.4%), norfloxacin (13.2%), chloramphenicol (59.4%) and trimethoprim/sulfamethoxazole (68.9%). When we investigated the sequence in the Blast database, the genome of the E. coli isolate indicated high similarity with the mcr-4 sequences. To our knowledge, this is the first report investigating on the mcr-4 gene in E. coli identified from cattle in Turkey. Our results highlighted that cattle might be a potential risk in transmitting mcr genes.
dc.description.sponsorshipSlovak Research and Development Agency [APVV-22-0457]; project VEGA [1-0162-23]
dc.description.sponsorshipThis work was supported by the Slovak Research and Development Agency under the Contract no. APVV-22-0457 and project VEGA no. 1-0162-23: Reduction in antibiotic use in dairy mastitis control programs .
dc.identifier.doi10.24425/pjvs.2024.151749
dc.identifier.endpage546
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue4
dc.identifier.orcid0000-0002-0449-0356
dc.identifier.orcid0000-0002-9173-9550
dc.identifier.pmid39736030
dc.identifier.scopus2-s2.0-85214188610
dc.identifier.scopusqualityQ3
dc.identifier.startpage537
dc.identifier.urihttps://doi.org/10.24425/pjvs.2024.151749
dc.identifier.urihttps://hdl.handle.net/11616/108779
dc.identifier.volume27
dc.identifier.wosWOS:001382076700006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn
dc.relation.ispartofPolish Journal of Veterinary Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectcattle
dc.subjectEscherichia coli
dc.subjectmcr
dc.subjectmultiplex PCR
dc.subjectNGS
dc.titleCarriage of antimicrobial resistance genes in Escherichia coli of bovine origin
dc.typeArticle

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