Genotoxic effects of chlorpyrifos, cypermethrin, endosulfan and 2,4-D on human peripheral lymphocytes cultured from smokers and nonsmokers

Basit Öğe Kaydı
dc.authorscopusid6602447584
dc.authorscopusid7006501958
dc.contributor.authorSandal S.
dc.contributor.authorYilmaz B.
dc.date.accessioned2024-08-04T20:02:26Z
dc.date.available2024-08-04T20:02:26Z
dc.date.issued2011
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPesticides often cause environmental pollution and adverse effects on human health. We have chosen four structurally different pesticides (endosulfan, an organochlorine pesticide; chlorpyrifos, an organophosphate insecticide; cypermethrin, type II pyrethroid insecticide, and 2,4-dichlorophenoxyacetic acid, a chlorinated aromatic hydrocarbon acid pesticide) to examine and compare their effects on DNA damage in acutely cultured human lymphocytes by the comet assay. In addition, possible differences in response between smoking and nonsmoking subjects were also investigated. Venous blood samples were obtained from healthy male nonsmoker (n = 7) and smoker (n = 8) donors. Primary cultures of lymphocytes were prepared and test groups were treated with three different concentrations (1, 5, and 10 ?M) of endosulfan, chlorpyrifos, cypermehrin, and 2,4-D. DNA damage was assessed by alkaline comet assay. We determined an increase in the ratio of DNA migration in human lymphocyte cell cultures as a result of treatment with cypermethrin, 2,4-D and chlorpyrifos at high concentration. Endosulfan had no significant genotoxic effect even at 10 ?M concentration. We suggest that chlorpyrifos and cypermethrin are more potentially genotoxic than endosulfan and 2,4-D. Our findings also indicate that the only significant DNA damage between smokers and nonsmokers was observed in the 2,4-D-treated group. © 2010 Wiley Periodicals, Inc.en_US
dc.identifier.doi10.1002/tox.20569
dc.identifier.endpage442en_US
dc.identifier.issn1520-4081
dc.identifier.issue5en_US
dc.identifier.pmid20196147en_US
dc.identifier.scopus2-s2.0-80052603544en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage433en_US
dc.identifier.urihttps://doi.org/10.1002/tox.20569
dc.identifier.urihttps://hdl.handle.net/11616/91698
dc.identifier.volume26en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofEnvironmental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChlorpyrifosen_US
dc.subjectCypermethrinen_US
dc.subjectEndosulfanen_US
dc.subjectGenotoxicityen_US
dc.subjectHuman lymphocytesen_US
dc.subjectSmoking and comet assayen_US
dc.titleGenotoxic effects of chlorpyrifos, cypermethrin, endosulfan and 2,4-D on human peripheral lymphocytes cultured from smokers and nonsmokersen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu