Hydrophobic microbeads as an alternative pseudo-affinity adsorbent for recombinant human interferon-? via hydrophobic interactions

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dc.authoridSAYLAN, YEŞEREN/0000-0001-5173-1522
dc.authoridUzun, Lokman/0000-0002-3971-7725
dc.authorwosid/ABI-3951-2020
dc.authorwosidDenizli, Adil/G-5151-2012
dc.authorwosidSAYLAN, YEŞEREN/HOF-7099-2023
dc.contributor.authorSaylan, Yeseren
dc.contributor.authorSari, Mufrettin Murat
dc.contributor.authorOzkara, Serpil
dc.contributor.authorUzun, Lokman
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-08-04T20:35:50Z
dc.date.available2024-08-04T20:35:50Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractHydrophobic interaction chromatography (HIC) is increasingly used for protein purification, separation and other biochemical applications. The aim of this study was to prepare hydrophobic microbeads and to investigate their recombinant human interferon-alpha (rHuIFN-alpha) adsorption capability. For this purpose, we had synthesized functional monomer, N-methacryloyl-L-phenylalanine (MAPA), to provide a hydrophobic functionality to the adsorbent. The poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-phenylalanine) [poly(HEMA-MAPA)] microbeads were prepared by suspension copolymerization. microbeads were characterized using FTIR, swelling behavior, and SEM micrographs. Equilibrium swelling ratio of poly(HEMA-MAPA) and poly(HEMA) microbeads were 53.3% and 69.3%, respectively. The specific surface area and average pore diameters determined by BET apparatus were 17.4 m(2)/g and 47.3 angstrom for poly(HEMA) microbeads and 18.7 m(2)/g and 49.8 angstrom for poly(HEMA-MAPA) microbeads. Adsorption experiments were performed under different conditions. Maximum rHuIFN-alpha adsorption capacity was found to be 137.6 +/- 6.7 mg/g by using poly(HEMA-MAPA) microbeads with a size range of 150-250 mu m and containing 327 mu mol MAPA/g microbeads. Compared with poly(HEMA-MAPA) microbeads, nonspecific rHuIFN-alpha adsorption onto plain poly(HEMA) microbeads was very low, about 4.2 +/- 2.3 mg/g. To determine the effects of adsorption conditions on possible conformational changes of rHuIFN-alpha structure, fluorescence spectrophotometry was employed. Repeated adsorption-elution processes showed that these microbeads are suitable for repeatable rHuIFN-alpha adsorption. (c) 2012 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.msec.2012.02.016
dc.identifier.endpage944en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84858284880en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage937en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2012.02.016
dc.identifier.urihttps://hdl.handle.net/11616/95598
dc.identifier.volume32en_US
dc.identifier.wosWOS:000303299300046en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMaterials Science & Engineering C-Materials For Biological Applicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRecombinant human interferon-alphaen_US
dc.subjectHydrophobic interaction chromatographyen_US
dc.subjectPhenylalanineen_US
dc.subjectPoly(HEMA-MAPA) microbeadsen_US
dc.subjectAffinity adsorptionen_US
dc.subjectPseudo-affinity adsorbentsen_US
dc.titleHydrophobic microbeads as an alternative pseudo-affinity adsorbent for recombinant human interferon-? via hydrophobic interactionsen_US
dc.typeArticleen_US

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