ILC3 deficiency and generalized ILC abnormalities in DOCK8-deficient patients

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dc.authoriduygun, vedat/0000-0003-3257-7798
dc.authoridEken, Ahmet/0000-0002-5816-0686
dc.authoridKiykim, Ayca/0000-0001-5821-3963
dc.authoridGuner, Sukru Nail/0000-0002-8860-6132
dc.authoridKeles, Sevgi/0000-0001-7344-8947
dc.authoridARTAC, Hasibe/0000-0002-9807-2605
dc.authoridKarakukcu, Musa/0000-0003-2015-3541
dc.authorwosiduygun, vedat/AGH-4534-2022
dc.authorwosidEken, Ahmet/AAK-4232-2021
dc.authorwosidArıkan Ayyildiz, Zeynep/ABE-9197-2021
dc.authorwosidKiykim, Ayca/J-6400-2017
dc.authorwosidGuner, Sukru Nail/A-9841-2018
dc.authorwosidAydiner, Elif/HKN-1599-2023
dc.authorwosidKeles, Sevgi/ACY-0399-2022
dc.contributor.authorEken, Ahmet
dc.contributor.authorCansever, Murat
dc.contributor.authorOkus, Fatma Zehra
dc.contributor.authorErdem, Serife
dc.contributor.authorNain, Ercan
dc.contributor.authorAzizoglu, Zehra Busra
dc.contributor.authorHaliloglu, Yesim
dc.date.accessioned2024-08-04T20:46:54Z
dc.date.available2024-08-04T20:46:54Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackgroundDedicator of cytokinesis 8 (DOCK8) deficiency is the main cause of the autosomal recessive hyper-IgE syndrome (HIES). We previously reported the selective loss of group 3 innate lymphoid cell (ILC) number and function in a Dock8-deficient mouse model. In this study, we sought to test whether DOCK8 is required for the function and maintenance of ILC subsets in humans. MethodsPeripheral blood ILC1-3 subsets of 16 DOCK8-deficient patients recruited at the pretransplant stage, and seven patients with autosomal dominant (AD) HIES due to STAT3 mutations, were compared with those of healthy controls or post-transplant DOCK8-deficient patients (n = 12) by flow cytometry and real-time qPCR. Sorted total ILCs from DOCK8- or STAT3-mutant patients and healthy controls were assayed for survival, apoptosis, proliferation, and activation by IL-7, IL-23, and IL-12 by cell culture, flow cytometry, and phospho-flow assays. ResultsDOCK8-deficient but not STAT3-mutant patients exhibited a profound depletion of ILC3s, and to a lesser extent ILC2s, in their peripheral blood. DOCK8-deficient ILC1-3 subsets had defective proliferation, expressed lower levels of IL-7R, responded less to IL-7, IL-12, or IL-23 cytokines, and were more prone to apoptosis compared with those of healthy controls. ConclusionDOCK8 regulates human ILC3 expansion and survival, and more globally ILC cytokine signaling and proliferation. DOCK8 deficiency leads to loss of ILC3 from peripheral blood. ILC3 deficiency may contribute to the susceptibility of DOCK8-deficient patients to infections.en_US
dc.identifier.doi10.1111/all.14081
dc.identifier.endpage933en_US
dc.identifier.issn0105-4538
dc.identifier.issn1398-9995
dc.identifier.issue4en_US
dc.identifier.pmid31596517en_US
dc.identifier.scopus2-s2.0-85074777767en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage921en_US
dc.identifier.urihttps://doi.org/10.1111/all.14081
dc.identifier.urihttps://hdl.handle.net/11616/99022
dc.identifier.volume75en_US
dc.identifier.wosWOS:000529134100017en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofAllergyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDOCK8en_US
dc.subjectHyper-IgE syndrome (HIES)en_US
dc.subjectILCen_US
dc.subjectILC3en_US
dc.subjectSTAT3en_US
dc.titleILC3 deficiency and generalized ILC abnormalities in DOCK8-deficient patientsen_US
dc.typeArticleen_US

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