Isolation of rosmarinic acid and methyl rosmarinate as lipoxygenase inhibitors from Salvia palaestina Benth. by activity-guided fractionation

dc.authoridBedir, Erdal/0000-0003-1262-063X
dc.authoridİçen, Mehmet Sina/0000-0002-1411-7184
dc.authoridDemirci, Fatih/0000-0003-1497-3017;
dc.authorwosidBedir, Erdal/ABI-3895-2020
dc.authorwosidİçen, Mehmet Sina/ABH-7505-2020
dc.authorwosidgurbuz, ilhan/AHB-3295-2022
dc.authorwosidDemirci, Fatih/A-8295-2008
dc.authorwosidGünbatan, Tuğba/AHA-9058-2022
dc.contributor.authorIcen, Mehmet Sina
dc.contributor.authorGurbuz, Ilhan
dc.contributor.authorBedir, Erdal
dc.contributor.authorGunbatan, Tugba
dc.contributor.authorDemirci, Fatih
dc.date.accessioned2024-08-04T20:50:16Z
dc.date.available2024-08-04T20:50:16Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractSalvia palaestina aqueous and methanol extracts were prepared from the aerial parts, which were evaluated for the in vitro anti-inflammatory properties using the lipoxygenase (LO) enzyme inhibition assay. While the aqueous extract showed no activity at test concentrations, a significant (p < 0.001) lipoxygenase inhibition was detected for the methanol extract with 29% inhibition. Activity guided fractionation was carried out on the methanol extract via liquid-liquid partitioning using n-hexane, dichloromethane, ethyl acetate, and n-butanol. The ethyl acetate fraction showed statistically the best inhibition among the sub-fractions with 70% inhibition (p < 0.0001). The compounds responsible for the activity were purified, and their structures were established as rosmarinic acid, and methyl rosmarinate by spectroscopic methods. IC50 values of rosmarinic acid, and methyl rosmarinate were calculated as 0.21 and 0.02 mu M, respectively. In conclusion, the in vitro anti-inflammatory potential of S. palaestina was associated to rosmarinic acid, and methyl rosmarinate, for the first time to the best of our knowledge. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.sajb.2021.04.030
dc.identifier.endpage182en_US
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.scopus2-s2.0-85106871246en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage177en_US
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2021.04.030
dc.identifier.urihttps://hdl.handle.net/11616/99959
dc.identifier.volume141en_US
dc.identifier.wosWOS:000752149500021en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSouth African Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSalvia palaestinaen_US
dc.subjectLipoxygenaseen_US
dc.subjectActivity-guided fractionationen_US
dc.subjectRosmarinateen_US
dc.subjectAnti-inflammatory activityen_US
dc.titleIsolation of rosmarinic acid and methyl rosmarinate as lipoxygenase inhibitors from Salvia palaestina Benth. by activity-guided fractionationen_US
dc.typeArticleen_US

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