High prevalence of TEM, VIM, and OXA-2 beta-lactamases and clonal diversity among Acinetobacter baumannii isolates in Turkey

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dc.authoridCELIK, Betul/0000-0002-0379-8076
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridAsgin, Nergis/0000-0001-9739-5675
dc.authorwosidCELIK, Betul/ABG-3981-2021
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidasgin, nergis/J-9292-2013
dc.contributor.authorAsgin, Nergis
dc.contributor.authorOtlu, Baris
dc.contributor.authorCakmakliogullari, Elcin Kal
dc.contributor.authorCelik, Betul
dc.date.accessioned2024-08-04T20:47:09Z
dc.date.available2024-08-04T20:47:09Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIntroduction: Acinetobacter baumannii is an opportunistic pathogen that causes nosocomial infections with high mortality. Treatment options are limited owing to its resistance to numerous antibiotics. Here, we sought to determine the antibiotic susceptibilities of A. baumannii isolates, investigate clonal relationship among the strains, and determine the frequency of beta-lactamase resistance genes. Methodology: The identification and antibiotic susceptibilities of 69 A. baumannii strains were determined using a BD-Phoenix automated system. The presence of bla(OXA-2), bla(OXA-10), bla(OXA-23), bla(OXA-24/)(40), bla(OXA-51), bla(OXA-58), bla(TEM), bla(SHV), bla(IMP), bla(VIM), and bla(GIM) genes were investigated using polymerase chain reaction (PCR), and clonal relatioships among the isolates were determined using pulsed-field gel electrophoresis (PFGE). Results: All strains were resistant to ampicillin-sulbactam, gentamicin, cefepime, ciprofloxacin, and ceftriaxone. While 65 of the 69 strains (94.2%) were resistant to piperacillin-tazobactam, amikacin, imipenem, and meropenem, all swains were susceptible to tigecycline and colistin. The frequencies of b/aoxik-51, blaoxA-23, bIa(TEM), bla(OXA-2), bla(VIM), and bla(SHV) were 100%, 94.2%, 53.6%, 21.7%, 14.5%, and 2.9%, respectively. Based on PFGE results, 56 of the 69 strains were clonally related, and the clustering rate was 81.2%. No common outbreak isolate was detected. Conclusions: The most prevalent OXA genes were bla(OXA-51), bla(OXA-23), and bla(OXA-2). Furthermore, bla(TEM), bla(SHV), and bla(VIM), which are common in Enterobacterales and Pseudomonas spp, were detected, suggesting horizontal gene transfer had occurred between bacteria. No single clone outbreak was detected by PFGE. However, multiclonal spread and the high clustering rate suggest cross-contamination. Therefore, in future, more effective infection control measures must be implemented.en_US
dc.description.sponsorshipKarabuk University Scientific Research Project Unit [KBU-BAP-KT-062]en_US
dc.description.sponsorshipThis study was funded by the Karabuk University Scientific Research Project Unit (project number: KBU-BAP-KT-062). The authors would like to thank Enago for the English language review.en_US
dc.identifier.doi10.3855/jidc.11684
dc.identifier.endpage801en_US
dc.identifier.issn1972-2680
dc.identifier.issue9en_US
dc.identifier.pmid32074088en_US
dc.identifier.scopus2-s2.0-85079738159en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage794en_US
dc.identifier.urihttps://doi.org/10.3855/jidc.11684
dc.identifier.urihttps://hdl.handle.net/11616/99192
dc.identifier.volume13en_US
dc.identifier.wosWOS:000490293200003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJ Infection Developing Countriesen_US
dc.relation.ispartofJournal of Infection in Developing Countriesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectbeta-lactamase genesen_US
dc.subjectoutbreaken_US
dc.subjectOXA-2en_US
dc.subjectOXA-23en_US
dc.subjectpulsed-field gel electrophoresisen_US
dc.titleHigh prevalence of TEM, VIM, and OXA-2 beta-lactamases and clonal diversity among Acinetobacter baumannii isolates in Turkeyen_US
dc.typeArticleen_US

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