Is hepatitis-B immunization effective during chronic liver fibrosis? Investigation of secretory and cellular immune responses on an experimental model

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dc.authoridKARACA, Zeynal Mete/0000-0002-5967-8102
dc.authoridKAYHAN, BASAK/0000-0003-3508-2563
dc.authoridYilmaz, Sezai/0000-0002-8044-0297
dc.authorwosidKARACA, Zeynal Mete/HKM-7924-2023
dc.authorwosidKAYHAN, BASAK/ABH-9314-2020
dc.authorwosidYilmaz, Sezai/ABI-2323-2020
dc.contributor.authorKayhan, Basak
dc.contributor.authorKaraca, Zeynal Mete
dc.contributor.authorCanpolat, Esra
dc.contributor.authorErsan, Veysel
dc.contributor.authorGul, Mehmet
dc.contributor.authorYologlu, Saim
dc.contributor.authorYilmaz, Sezai
dc.date.accessioned2024-08-04T20:53:02Z
dc.date.available2024-08-04T20:53:02Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective Adults with end-stage of chronic liver diseases have lower antibody titers after hepatitis-B vaccination. We have less amount of knowledge about the effect of non-viral cause chronic liver fibrosis on vaccination. In this study, we investigated the effect of non-viral chronic liver fibrosis on hepatitis B vaccine and the effect of tetanous toxoid co-administration at the level of humoral and cellular immune responses in an experimental model. Methods Hepatitis B vaccine was administered either alone or in combination with tetanus toxoid in thioacetamide-induced fibrotic BALB/c mice. Fibrosis level was determined by Knodell scoring. Anti-HBsAg, biochemical parameters, inflammatory (IL-1 beta, TNF-alpha), and anti-inflammatory (IL-10) cytokine levels were investigated in serum samples by automated systems and ELISA; respectively. Frequencies of activated lymphocytes were determined in flow cytometer. Results Antibody titers significantly decreased after immunization of fibrotic mice. However, co-administration of toxoid significantly elevated antibody titer. The percentage of CD19(+)CD69(+) B lymphocytes was found to be lower in vaccinated fibrotic group compared to vaccinated naive group. Simultaneous administration of toxoid significantly increased the frequencies of CD4(+) and CD8(+) T cells expressing CD69 and CD127. Interestingly, CD19(+)CD5(+)CD1(high) Breg cells were significantly reduced in the group vaccinated with hepatitis B vaccine and toxoid, simultaneously. The reduction in Breg percentage did not expose a significant decrease in the level of IL-10. Conclusion Non-viral chronic liver fibrosis causes a reduction on specific antibody level after vaccination. Reduction on Breg cell frequency may have an effect on elevation of antibody level after co-administration of tetanus toxoid.en_US
dc.description.sponsorshipInonu University Scientific Research Projects Unit [2016/137]en_US
dc.description.sponsorshipThis work was supported by the Inonu University Scientific Research Projects Unit under grant number 2016/137.en_US
dc.identifier.doi10.1080/08923973.2022.2121925
dc.identifier.endpage113en_US
dc.identifier.issn0892-3973
dc.identifier.issn1532-2513
dc.identifier.issue1en_US
dc.identifier.pmid36066099en_US
dc.identifier.scopus2-s2.0-85138356832en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage102en_US
dc.identifier.urihttps://doi.org/10.1080/08923973.2022.2121925
dc.identifier.urihttps://hdl.handle.net/11616/100899
dc.identifier.volume45en_US
dc.identifier.wosWOS:000853788700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofImmunopharmacology and Immunotoxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLiver fibrosisen_US
dc.subjectvaccinationen_US
dc.subjecttoxoiden_US
dc.subjectantibodyen_US
dc.subjectBregen_US
dc.titleIs hepatitis-B immunization effective during chronic liver fibrosis? Investigation of secretory and cellular immune responses on an experimental modelen_US
dc.typeArticleen_US

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