Real-time cell analysis of the cytotoxicity of orthodontic mini-implants on human gingival fibroblasts and mouse osteoblasts

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dc.authoridHakkı, Sema S/0000-0001-8665-6235
dc.authorwosidHakkı, Sema S/Y-2878-2018
dc.contributor.authorMalkoc, Siddik
dc.contributor.authorOzturk, Firat
dc.contributor.authorCorekci, Bayram
dc.contributor.authorBozkurt, Buket S.
dc.contributor.authorHakki, Sema S.
dc.date.accessioned2024-08-04T20:35:50Z
dc.date.available2024-08-04T20:35:50Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIntroduction: The aim of this study was to evaluate the cytotoxic effects of orthodontic mini-implants on gingival fibroblasts and osteoblasts. Methods: The orthodontic mini-implants used in this study were Orthodontic Mini Implant (Leone, Florence, Italy), MTN (MTN, Istanbul, Turkey), AbsoAnchor (Dentos, Daegu, South Korea), IMTEC Ortho (3M Unitek, IMTEC, Ardmore, Okla), VectorTAS (Ormco, Glendora, Calif). The materials were incubated in Dulbecco's modified eagle's culture medium for 72 hours according to ISO 10993-5 standards (surface area-to-volume ratio of the specimen to cell-culture medium, 3 cm(2)/mL). A real-time cell analyzer (xCELLigence, Roche Applied Science, Mannheim, Germany; ACEA Biosciences, San Diego, Calif) was used to evaluate cell survival. After seeding 200 mu L of the cell suspensions into the wells of the E-plate 96, gingival fibroblasts were treated with bioactive components released by the metallic materials and monitored every 15 minutes for 190 hours. For the proliferation experiments, the statistical analyses used were 1-way analysis of variance and Tukey-Kramer multiple comparisons tests. Results: There was no significant differences between the human gingival fibroblast cell indexes of the control and study groups (P>0.05). When evaluated at 27 and 96 hours, only the VectorTAS mini-implants showed statistically significant decreases in the M3T3 cell index (P < 0.001) compared with the control group. No significant differences were found among the control and all study groups (P>0.05). Furthermore, the Leone and MTN mini-implants showed statistically significant decreases (P < 0.001) at 190 hours. Also, the VectorTAS mini-implants demonstrated a significant decline (P < 0.05) at the same time in the M3T3 cell index. Conclusions: These findings provide fundamental knowledge and new insights for future design and development of new biocompatible titanium alloys for orthodontic mini-implants and temporary anchorage devices. (Am J Orthod Dentofacial Orthop 2012;141:419-26)en_US
dc.identifier.doi10.1016/j.ajodo.2011.12.009
dc.identifier.endpage426en_US
dc.identifier.issn0889-5406
dc.identifier.issn1097-6752
dc.identifier.issue4en_US
dc.identifier.pmid22464523en_US
dc.identifier.scopus2-s2.0-84859168762en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage419en_US
dc.identifier.urihttps://doi.org/10.1016/j.ajodo.2011.12.009
dc.identifier.urihttps://hdl.handle.net/11616/95606
dc.identifier.volume141en_US
dc.identifier.wosWOS:000302133100013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMosby-Elsevieren_US
dc.relation.ispartofAmerican Journal of Orthodontics and Dentofacial Orthopedicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIn-Vitro Evaluationen_US
dc.subjectTitanium-Alloyen_US
dc.subjectAnchorageen_US
dc.subjectCorrosionen_US
dc.subjectParticlesen_US
dc.titleReal-time cell analysis of the cytotoxicity of orthodontic mini-implants on human gingival fibroblasts and mouse osteoblastsen_US
dc.typeArticleen_US

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