Genetic diversity and antibiotic resistance profiles of Campylobacter jejuni isolates from poultry and humans in Turkey

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dc.authoridHIZLISOY, Harun/0000-0003-3391-0185
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridERTAS ONMAZ, Nurhan/0000-0002-4679-6548;
dc.authorwosidkayman, tuba/JQJ-4816-2023
dc.authorwosidHIZLISOY, Harun/E-2333-2019
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidAydın, Fuat/AAM-5216-2021
dc.authorwosidHIZLISOY, Harun/AAL-8869-2021
dc.authorwosidERTAS ONMAZ, Nurhan/GXH-7190-2022
dc.authorwosidABAY, Seçil/AAI-9526-2021
dc.contributor.authorAbay, Secil
dc.contributor.authorKayman, Tuba
dc.contributor.authorOtlu, Baris
dc.contributor.authorHizlisoy, Harun
dc.contributor.authorAydin, Fuat
dc.contributor.authorErtas, Nurhan
dc.date.accessioned2024-08-04T20:38:04Z
dc.date.available2024-08-04T20:38:04Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, the investigation of clonal relations between human and poultry Campylobacter jejuni isolates and the determination of susceptibilities of isolates to various antibiotics were aimed. A total of 200 C. jejuni isolates concurrently obtained from 100 chicken carcasses and 100 humans were genotyped by the Pulsed-Field Gel Electrophoresis (PFGE) and automated Repetitive Extragenic Palindromic PCR (Rep-PCR, DiversiLab system) methods and were tested for their susceptibility to six antibiotics with disk diffusion method. The minimum inhibitory concentration (MIC) values of ciprofloxacin (CI), enrofloxacin (EF) and erythromycin (EM) were evaluated by E-test. By using PFGE 174 of (87.0%) the isolates were able to be typed. The clonally related strains were placed in 35 different clusters and 115 different genotypes were obtained. All of the two hundred isolates could be typed by using Rep-PCR and were divided into 133 different genotypes. One hundred and fourteen clonally related isolates (57.0%) were included in 47 clusters. In disk diffusion test, while the susceptibility rates of AMC and S to human and chicken derived C jejuni isolates were 84.0%-96.0% and 96.0%-98.0%, respectively, all isolates were susceptible to gentamicin. The resistance rates of human isolates to AMP, NA and TE were detected as 44.0%, 84.0% and 38.0% of the resistances of chicken isolates to these antibiotics were 34.0%, 95.0% and 56.0%, respectively. The MIC values of human and chicken isolates to Cl, EF and EM were detected as 81.0-93.0%, 85.0-88.0% and 6.0-7.0%, respectively. The clonal proximity rates were detected between human and poultry origin C. jejuni isolates. The discriminatory power of PFGE and Rep-PCR was similar, with Simpson's diversity indexes of 0.993 and 0.995, respectively. Concordance of the two methods as determined by Adjusted Rand coefficient was 0.198 which showed the low congruence between Rep-PCR and PFGE. High rates of quinolone resistance were detected in C. jejuni isolates. This study demonstrated that chicken meat played an important role for infections caused by C. jejuni in Turkey and erythromycin, amoxicillin clavulanic acid and gentamicin are recommended for the treatment of Campylobacteriosis in humans. (c) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific Research Council of Erciyes University, Kayseri, Turkey [TSA-11-3414]en_US
dc.description.sponsorshipThis research was supported by the Scientific Research Council of Erciyes University, Kayseri, Turkey (Project No. TSA-11-3414).en_US
dc.identifier.doi10.1016/j.ijfoodmicro.2014.03.003
dc.identifier.endpage38en_US
dc.identifier.issn0168-1605
dc.identifier.issn1879-3460
dc.identifier.pmid24667316en_US
dc.identifier.scopus2-s2.0-84896460329en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage29en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijfoodmicro.2014.03.003
dc.identifier.urihttps://hdl.handle.net/11616/96364
dc.identifier.volume178en_US
dc.identifier.wosWOS:000335708100005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Food Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibiogramen_US
dc.subjectCampylobacter jejunien_US
dc.subjectPulsed-Field Gel Electrophoresisen_US
dc.subjectRepetitive extragenic palindromic-polymerase chain reactionen_US
dc.titleGenetic diversity and antibiotic resistance profiles of Campylobacter jejuni isolates from poultry and humans in Turkeyen_US
dc.typeArticleen_US

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