Cytokine profile and nitric oxide levels in sera from patients with brucellosis

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dc.authoridErsoy, Yasemin/0000-0001-5730-6682
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidErsoy, Yasemin/AAE-4811-2020
dc.contributor.authorRefik, M
dc.contributor.authorMehmet, N
dc.contributor.authorDurmaz, R
dc.contributor.authorErsoy, Y
dc.date.accessioned2024-08-04T20:56:13Z
dc.date.available2024-08-04T20:56:13Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe aims of this study were to investigate the serum levels of some cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), IL-2R, IL-6, and IL-8] and nitric oxide (NO) levels in patients with untreated brucellosis and to test the correlation of these parameters with each other. The study was conducted on 67 subjects, 37 patients with brucellosis and 30 healthy individuals with no history of Brucella infection. Brucellosis was identified by a positive blood culture and/or increased Brucella antibodies in serological tests in addition to compatible clinical symptoms. Cytokine profile analysis was performed by the immulite chemiluminescent enzyme immunometric assay whose inter- and intra-assay coefficients of variance were 2.6-3.6 and 4.4-8.5%, respectively. The levels of nitrites/nitrates, which are representative of NO levels, were measured by the Griess method. Patients with brucellosis had significantly elevated serum levels of nitrites/nitrates, IL-2R, IL-6 and IL-8 (mean +/- SD, 102.8 +/- 23.8 mumol/l, 806.1 +/- 58.5 U/ml, 21.1 +/- 2.3 mug/ml, and 8.8 +/- 1.6 pg/ml, respectively) compared to healthy controls, whereas TNF-alpha and IL-1beta levels were unchanged. No statistically significant correlation was detected between any of the studied cytokine levels and nitrate/nitrite concentrations according to Pearson's linear correlation test. We conclude that only IL-6, IL-8 and IL-2R are elevated in brucellosis and the extent of elevation depends on the severity and clinical pattern of the disease. Moderate elevation in serum NO was comparable to that observed in previous studies. This explains the absence or very rare occurrence of septic shock in brucellosis.en_US
dc.identifier.doi10.1590/S0100-879X2004001100010
dc.identifier.endpage1663en_US
dc.identifier.issn0100-879X
dc.identifier.issn1678-4510
dc.identifier.issue11en_US
dc.identifier.pmid15517081en_US
dc.identifier.scopus2-s2.0-9244242629en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1659en_US
dc.identifier.urihttps://doi.org/10.1590/S0100-879X2004001100010
dc.identifier.urihttps://hdl.handle.net/11616/102140
dc.identifier.volume37en_US
dc.identifier.wosWOS:000225128700010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAssoc Bras Divulg Cientificaen_US
dc.relation.ispartofBrazilian Journal of Medical and Biological Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjecthuman brucellosisen_US
dc.subjectcytokinesen_US
dc.subjectnitric oxideen_US
dc.subjectinterleukin-2en_US
dc.subjectinterleukin-6en_US
dc.subjectinterleukin-8en_US
dc.titleCytokine profile and nitric oxide levels in sera from patients with brucellosisen_US
dc.typeArticleen_US

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