Boron stress signal is transmitted through the TOR pathway

Basit Öğe Kaydı
dc.contributor.authorYilmaz, Irem Uluisik
dc.contributor.authorKoc, Ahmet
dc.date.accessioned2024-08-04T20:53:45Z
dc.date.available2024-08-04T20:53:45Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAlthough boron is an essential element for many organisms, an excess amount of it can cause toxicity, and the mechanism behind this toxicity is not yet fully understood. The Gcn4 transcription factor plays a crucial role in the boron stress response by directly activating the expression of the boron efflux pump Atr1. More than a dozen transcription factors and multiple cell signaling pathways have roles in regulating the Gcn4 transcription factor under various circumstances. However, it is unknown which pathways or factors mediate boron signaling to Gcn4. Using the yeast Saccharomyces cerevisiae as a model, we analyzed the factors that converge on the Gcn4 transcription factor to assess their possible roles in boron stress signaling. Our findings show that the GCN system is activated by uncharged tRNA stress in response to boron treatment and that GCN1, which plays a role in transferring uncharged tRNAs to Gcn2, is necessary for the kinase activity of Gcn2. The SNF and PKA pathways were not involved in mediating boron stress, even though they interact with Gcn4. Mutations in TOR pathway genes, such as GLN3 and TOR1, abolished Gcn4 and ATR1 activation in response to boric acid treatment. Therefore, our study suggests that the TOR pathway must be functional to form a proper response against boric acid stress.en_US
dc.description.sponsorshipTUBITAK [110T917]en_US
dc.description.sponsorshipAcknowledgments We would like to express our gratitude to C;ag?lar Karakaya for his assistance in this study. This work was supported by TUBITAK grant no. 110T917 (AK) .en_US
dc.identifier.doi10.1016/j.jtemb.2023.127222
dc.identifier.issn0946-672X
dc.identifier.issn1878-3252
dc.identifier.pmid37270859en_US
dc.identifier.scopus2-s2.0-85160618379en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jtemb.2023.127222
dc.identifier.urihttps://hdl.handle.net/11616/101378
dc.identifier.volume79en_US
dc.identifier.wosWOS:001012726100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofJournal of Trace Elements in Medicine and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBoric aciden_US
dc.subjectBoron stressen_US
dc.subjectYeasten_US
dc.subjectGeneral amino acid controlen_US
dc.subjectTor pathwayen_US
dc.subjectGcn1en_US
dc.titleBoron stress signal is transmitted through the TOR pathwayen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu