Investigation of the relationship between nitric oxide metabolites' levels and adenosine deaminase activity in 7,12-dimethylbenz[a]anthracene induced mouse liver

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dc.authoridBatcioglu, Kadir/0000-0001-6663-2287
dc.authorwosidBatcioglu, Kadir/Q-2424-2015
dc.contributor.authorOzturk, IC
dc.contributor.authorBatcioglu, K
dc.date.accessioned2024-08-04T20:14:43Z
dc.date.available2024-08-04T20:14:43Z
dc.date.issued2002
dc.departmentİnönü Üniversitesien_US
dc.description.abstract7,12-Dimethylbenz[a]anthracene (7,12DMBA) is a member of the polycyclic aromatic hydrocarbons with a severe carcinogenic effect. In this study, nitrate levels and ADA (Adenosine deaminase) activity in the liver homogenates of mice were measured and the effect of free radicals induced by 7,12-DMBA on inducible nitric oxide synthase (iNOS) and ADA activity were investigated. Antioxidant effects of melatonin were also compared. Three groups of mice were included in the study. The first served as control, the second was treated only with 7,12-DMBA and the third was treated with 7,12-DMBA+melatonin. Spectrophotometric methods were used at all measurements. Data were analyzed using Kruskal-Wallis Variance Analysis Test and Mann-Whitney U Test that were applied to the groups. The nitrate concentrations of mouse liver were as follows: 4.98 +/- 0.63 mumol/L in the control group (n = 10), 8.23 +/- 1.58 mumol/L (higher than control group, p < 0.05) in the 7,12-DMBA-treated group (n = 10), and 6.43 +/- 0.57 mumol/L (lower than 7,12-DMBA-treated group, p < 0.05) in the 7,12-DMBA+melatonintreated group (n = 10). Liver ADA activities were measured to be 4.14 +/- 0.674 U/L in the control group, 6.25 +/- 1.261 U/L (higher than control group, p < 0.05) in the 7,12-DMBA-treated group, and 4.93 +/- 0.916 U/L (lower than 7,12-DMBA-treated group, p < 0.05) in the 7,12-DMBA+melatonin-treated group. Differences between free nitrite levels were no significantly. Results demonstrated that nitrate levels and ADA activities were increased by means of free radicals induced by 7,12-DMBA. Melatonin inhibited the 7,12DMBA induced increase that was observed in the activities of ADA enzyme and nitrate levels. It is concluded that determination of ADA activity and nitrate levels can be useful in the assesment of liver damage caused by toxic chemicals. (C) 2002 Wiley Periodicals, Inc.en_US
dc.identifier.doi10.1002/jbt.10041
dc.identifier.endpage262en_US
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue5en_US
dc.identifier.pmid12439868en_US
dc.identifier.scopus2-s2.0-1842830974en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage260en_US
dc.identifier.urihttps://doi.org/10.1002/jbt.10041
dc.identifier.urihttps://hdl.handle.net/11616/93917
dc.identifier.volume16en_US
dc.identifier.wosWOS:000179584600007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Biochemical and Molecular Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectadenosine deaminaseen_US
dc.subjectnitric oxide metabolitesen_US
dc.subject7,12-DMBAen_US
dc.subjectliver damageen_US
dc.subjectmelatoninen_US
dc.titleInvestigation of the relationship between nitric oxide metabolites' levels and adenosine deaminase activity in 7,12-dimethylbenz[a]anthracene induced mouse liveren_US
dc.typeArticleen_US

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