A new approach on the regulation of NF-?B and Bax protein signaling pathway activation by royal jelly in fluoride-induced pancreas damage in rats

Basit Öğe Kaydı
dc.authoridGök, Özlem/0000-0001-8521-6369
dc.authoridParlak, Gozde/0000-0002-8982-887X
dc.authoridCan, Muhammed Ismail/0000-0002-0118-2278
dc.authorwosidGök, Özlem/AFV-8223-2022
dc.authorwosidParlak, Gozde/ISA-6808-2023
dc.authorwosidAslan, Abdullah/C-9523-2016
dc.authorwosidcan, mi/KDN-4165-2024
dc.authorwosidCan, Muhammed İsmail/ABG-8646-2020
dc.contributor.authorAslan, Abdullah
dc.contributor.authorCan, Muhammed Ismail
dc.contributor.authorBeyaz, Seda
dc.contributor.authorGok, Ozlem
dc.contributor.authorParlak, Gozde
dc.contributor.authorGundogdu, Ramazan
dc.contributor.authorOzercan, Ibrahim Hanifi
dc.date.accessioned2024-08-04T20:52:18Z
dc.date.available2024-08-04T20:52:18Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractForty-two healthy adult male rats (Wistar albino, n = 42, 8 weeks old, starting weights 200-250 g) employed in this study were subdivided into six groups randomly with seven rats per group as follows: (i) Control group: received standard diet; (ii) RJ group: received standard diet supplemented with royal jelly; (iii) F50 group: received standard diet supplemented with fluoride (50 mg/kg BW); (iv) F100 group: received standard diet supplemented with fluoride (100 mg/kg BW); (v) F50 +RJ group: received standard diet supplemented with fluoride (50 mg/kg BW) and royal jelly; (iv) F100 +RJ group: received standard diet supplemented with fluoride (100 mg/kg BW) and royal jelly. The study continued for a total of eight weeks. Western blot analysis was conducted to determine the post-translational expression levels of NF-kappa B, Bax, Bcl-2, TNF-alpha, Caspase-3 and Caspase-6 proteins in pancreas tissue. The pancreatic tissue was subjected to histopathological evaluation. Furthermore, MDA, GSH and CAT activities were examined by spectrophotometric analyzes. Our findings demonstrate that, compared to the control and RJ groups, Bcl-2 protein expression was augmented and, conversely, Caspase-6, Caspase-3 and Bax protein levels were decreased upon fluoride treatment. A statistically significant increase in TNF-alpha and NF-kappa B protein expressions was observed in the groups with fluoride-induced damage compared to the control and RJ groups. The MDA levels were increased in all fluoride-treated rats compared to those in the control and RJ groups, whereas the CAT and GSH activities were reduced in all rats with fluoride-induced damage. Although there was not a great difference between the groups regarding histopath-ological findings, there was a tendency to decrease in the rate of damage upon royal jelly treatment.en_US
dc.identifier.doi10.1016/j.tice.2022.101913
dc.identifier.issn0040-8166
dc.identifier.pmid36095934en_US
dc.identifier.scopus2-s2.0-85137581515en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.tice.2022.101913
dc.identifier.urihttps://hdl.handle.net/11616/100878
dc.identifier.volume79en_US
dc.identifier.wosWOS:000862197900002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofTissue & Cellen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRoyal jellyen_US
dc.subjectPancreas damageen_US
dc.subjectCaspase-3en_US
dc.subjectCaspase-6en_US
dc.subjectBaxen_US
dc.titleA new approach on the regulation of NF-?B and Bax protein signaling pathway activation by royal jelly in fluoride-induced pancreas damage in ratsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu