THE EFFECTS OF EXOGENOUS ADRENOMEDULLIN AND L-NAME ON ANTIOXIDANT DEFENCE SYSTEM OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS AND LIPID PARAMETERS OF SERUM IN RAT
Küçük Resim Yok
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of our study was to investigate the contribution of exogenous adrenomedullin (ADM): an antioxidant, and N sup omega nitro-L-arginine methyl ester (L-NAME); the nitric oxide synthase (NOS) enzyme inhibitor, to balance the oxidant/antioxidant status in hypothalamus and adrenal medulla, which are both key components of the hypothalamic-pituitary-adrenal (HPA) axis and affects some lipid metabolic mediators. Thirty six male albino Spraque-Dawley rats were randomly divided into three groups: the control group, the ADM group (8 nmol/kg, i.p.), and the L-NAME group (2 ml/kg, i.p.). Measurements of tissue superoxide dismutase (SOD), catalase (CAT), glutathion S-transferase (GST) and glutathion reductase (GR) activities and glutathione (GSH) were performed in tissues using chemical protocols. The biochemical analyses for total lipid, cholesterol, triglycerides, HDL, VLDL, and LDL were conducted on an auto-analyser. Administering exogenous ADM and L-NAME caused synergic changes in the tissues, antioxidative enzyme activities, and altered serum lipoprotein profiles in rats. In the adrenal medulla and hypothalamus of the experimental groups, SOD and GST activities decreased significantly, while the CAT and GR activities increased. The amount of GSH was enhanced in the both tissues in the ADM group only, while the amount was decreased for the hypothalamus of the L-NAME group. After the treatment, the cholesterol (of L-NAME group), triglyceride, total lipid, and VLDL parameters varied significantly, but there was no significant difference between the HDL and LDL among the groups.
Açıklama
Anahtar Kelimeler
Adrenomedullin, L-NAME, glutathione, antioxidant enzymes, lipid metabolism, rat
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
20
Sayı
8
