Comparison of Six Aptamer-Aptamer Pairs on Rapid Detection of SARS-CoV-2 by Lateral Flow Assay

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dc.authoridOtlu, Baris/0000-0002-6220-0521
dc.contributor.authorDerin, Dilek Cam
dc.contributor.authorGultekin, Enes
dc.contributor.authorGunduz, Elif
dc.contributor.authorOtlu, Baris
dc.date.accessioned2024-08-04T20:55:57Z
dc.date.available2024-08-04T20:55:57Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground SARS-CoV-2 is a threat to humanity. Both the spike (S) protein and its receptor binding domain (sRBD) are extensively used for rapid detection. Although real-time reverse transcription polymerase chain reaction (rRT-PCR) is mostly used method for the molecular detection of SARS-CoV-2, rapid assays for antigenic detection are always needed. Lateral flow assays (LFAs) are the most commonly used tests for this purpose, and aptamers having stability and long shelf life are used as capture reagents.Objective This study aimed to develop the LFAs based on the aptamer pairs for the antigenic detection of SARS-CoV-2 with the naked eye.Method Gold nanoparticles (AuNPs) were used as labels, and six sandwich models by three different aptamers were prepared using 4 mu M and 8 mu M probes and two kinds of membranes for developing the LFAs.Results The 8 mu M probe concentration and M2 membrane showed the best recognition of both the synthetic sRBD and SARS-CoV-2 coming from the naso/oropharingeal swabs by designed LFAs as 100% sensitivity and 93.3% specificity compared to the antibody-detecting LFAs.Conclusions Our developed strip assays based on aptamer pairs recognized the target directly in 5-6 min with the naked eye. It was also concluded that aptamer pairs, membrane types, assay buffers, and probe concentrations have a significant role in the detection of SARS-CoV-2 by LFAs.Highlights The detection of SARS-CoV-2 in clinical samples was demonstrated with the best aptamer pairs, sensitively and selectively among the designed six aptamer pairs for LFAs. Developed LFAs can be an alternative method to the conventional antibody-based LFAs for SARS-CoV-2 detection.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [120Z962]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK; 120Z962).en_US
dc.identifier.doi10.1093/jaoacint/qsae004
dc.identifier.endpage470en_US
dc.identifier.issn1060-3271
dc.identifier.issn1944-7922
dc.identifier.issue3en_US
dc.identifier.pmid38218729en_US
dc.identifier.scopus2-s2.0-85192114125en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage464en_US
dc.identifier.urihttps://doi.org/10.1093/jaoacint/qsae004
dc.identifier.urihttps://hdl.handle.net/11616/101963
dc.identifier.volume107en_US
dc.identifier.wosWOS:001175518900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOxford Univ Press Incen_US
dc.relation.ispartofJournal of Aoac Internationalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSpike Proteinen_US
dc.subjectDiscoveryen_US
dc.titleComparison of Six Aptamer-Aptamer Pairs on Rapid Detection of SARS-CoV-2 by Lateral Flow Assayen_US
dc.typeArticleen_US

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