Comparative evaluation of cytotoxic and anti-metastatic function of microbial chondroitin sulfate and animal-originated commercial chondroitin sulfate in cancer cells

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dc.authoridUnver, Tuba/0000-0002-8655-2716
dc.authorwosidUnver, Tuba/ABI-6440-2020
dc.contributor.authorUnver, Tuba
dc.contributor.authorCelik-Uzuner, Selcen
dc.contributor.authorErenler, Ayse Sebnem
dc.date.accessioned2024-08-04T20:54:47Z
dc.date.available2024-08-04T20:54:47Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractCancer has the second-highest mortality rate worldwide after cardiovascular disease. In addition, cervical and breast cancer are two of the leading causes of cancer-related deaths among women. The tumor microenvironment, which consists of cells that form blood vessels, proteins, fibroblasts, and immune cells, is a therapeutic target for cancer therapy. As part of the extracellular matrix (ECM), glycosaminoglycan Chondroitin Sulfate (CS) is related to diverse aspects of tumor growth and metastasis depending on the CS sulfate pattern. This study analyzed the roles of Microbial CS and Commercial CS in tumor growth and metastasis using HeLa cervical cancer cells, MDA-MB-231 metastatic breast cancer cells, and normal fibroblasts. In addition, the role of CS types in wound healing was also assessed comparatively. Microbial CS was more cytotoxic in MDA-MB-231 cells than HeLa compared to Commercial CS. Although both CS reduced cell viability in normal cells, the selective index of Microbial CS in MDA-MB-213 cells was higher than its commercial counterpart. In addition, the role of CS types in wound healing was also assessed comparatively. Both types of CS decreased the cell migration in MDA-MB-231 breast cancer cells, but HeLa cells were more sensitive to Microbial CS than Commercial CS to heal the wound. The wound healing of NIH3T3 cells after Microbial CS was similarly high to the healing after Commercial CS. This preliminary study shows that microbial CS produced by biotechnological methods from a recombinant source created by our team can be an effective therapeutic agent in various types of cancer.en_US
dc.identifier.doi10.14715/cmb/2023.69.9.2
dc.identifier.endpage14en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue9en_US
dc.identifier.pmid37807340en_US
dc.identifier.scopus2-s2.0-85175431485en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage8en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2023.69.9.2
dc.identifier.urihttps://hdl.handle.net/11616/101644
dc.identifier.volume69en_US
dc.identifier.wosWOS:001116557900008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicrobial Chondroitin Sulfateen_US
dc.subjectAnimal-originated Chondroitin Sulfateen_US
dc.subjectanticancer agenten_US
dc.subjectanti-metastatic agenten_US
dc.subjectwound healingen_US
dc.titleComparative evaluation of cytotoxic and anti-metastatic function of microbial chondroitin sulfate and animal-originated commercial chondroitin sulfate in cancer cellsen_US
dc.typeArticleen_US

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