Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes

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dc.authoridBoran, Filiz Kuru/0000-0002-8801-7987
dc.authoridYesilada, Ozfer/0000-0003-0038-6575
dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authorwosidBirhanli, Emre/AAA-4009-2021
dc.authorwosidBoran, Filiz Kuru/AAA-3061-2021
dc.authorwosidKöytepe, Süleyman/AAA-4168-2021
dc.authorwosidYesilada, Ozfer/ABI-1335-2020
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorUlu, Ahmet
dc.contributor.authorBirhanli, Emre
dc.contributor.authorBoran, Filiz
dc.contributor.authorKoytepe, Suleyman
dc.contributor.authorYesilada, Ozfer
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:47:08Z
dc.date.available2024-08-04T20:47:08Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, a novel immobilization support for laccase was developed to enhance enzyme stability, efficiency and reusability. Firstly, Fe3O4 magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS). The support was characterized using several methods. Afterward, laccase was attached to the surface of functional support. The biochemical properties of the immobilized laccase were comprehensively investigated. It was observed that immobilized laccase achieved maximum activity at pH 4.0 and the optimum temperature was found to be 50 degrees C. After storage at +4 degrees C and similar to 25 degrees C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively. At 55 degrees C, the activity of immobilized lactase decreased to 73A% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity. Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity. These results are promising for the use of laccase in industrial and biotechnological applications. Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme. (C) 2020 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.ijbiomac.2020.02.006
dc.identifier.endpage884en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid32027899en_US
dc.identifier.scopus2-s2.0-85079541328en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage871en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2020.02.006
dc.identifier.urihttps://hdl.handle.net/11616/99184
dc.identifier.volume150en_US
dc.identifier.wosWOS:000525869500086en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectThiolated chitosanen_US
dc.subjectMagnetic nanopartidesen_US
dc.subjectLaccaseen_US
dc.subjectImmobilizationen_US
dc.subjectTextile dyeen_US
dc.subjectDecolorizationen_US
dc.titleLaccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyesen_US
dc.typeArticleen_US

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