Genetic engineering of Enterobacter aerogenes with the Vitreoscilla hemoglobin gene

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dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authoridKahraman, Hüseyin/0000-0001-6235-5497
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.authorwosidKahraman, Hüseyin/ABG-9650-2020
dc.contributor.authorGeckil, H
dc.contributor.authorGencer, S
dc.contributor.authorKahraman, H
dc.contributor.authorErenler, SO
dc.date.accessioned2024-08-04T20:13:23Z
dc.date.available2024-08-04T20:13:23Z
dc.date.issued2003
dc.departmentİnönü Üniversitesien_US
dc.description.abstractHemoglobins in unicellular organisms, like the one here in the bacterium Vitreoscilla, have greater chemical reactivity than their homologues in multicellular organisms. They can catalyze redox reactions and may protect cells against oxidative stress. The ability of Vitreoscilla hemoglobin to complement deficiencies of terminal cytochrome oxidases in Escherichia coli also suggests that this hemoglobin can receive electrons during respiration. In this study, a recombinant strain of Enterobacter aerogenes engineered to produce the Vitreoscilla Hb was investigated with regard to its susceptibility to oxidative stress. The culture response to oxidative stress produced by exogenously applied hydrogen peroxide was characterized in terms of cell growth, survival and the activities of two key antioxidant enzymes (catalase and superoxide dismutase). The influence of the physiological state of the cells and different media upon these culture dynamics was determined. Results showed that the hemoglobin-expressing strain is quite distinct in terms of growth/survival properties and activity of antioxidant enzymes from that of non-hemoglobin counterparts. (C) 2003 Editions scientitiques et medicales Elsevier SAS. All rights reserved.en_US
dc.identifier.doi10.1016/S0923-2508(03)00083-4
dc.identifier.endpage431en_US
dc.identifier.issn0923-2508
dc.identifier.issn1769-7123
dc.identifier.issue6en_US
dc.identifier.pmid12892849en_US
dc.identifier.scopus2-s2.0-0041706293en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage425en_US
dc.identifier.urihttps://doi.org/10.1016/S0923-2508(03)00083-4
dc.identifier.urihttps://hdl.handle.net/11616/93585
dc.identifier.volume154en_US
dc.identifier.wosWOS:000185313100006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofResearch in Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbacterial hemoglobinen_US
dc.subjectVitreoscilla hemoglobinen_US
dc.subjectoxygen uptakeen_US
dc.subjectcatalaseen_US
dc.subjectsuperoxide dismutaseen_US
dc.subjectoxidative stressen_US
dc.subjecthydrogen peroxideen_US
dc.titleGenetic engineering of Enterobacter aerogenes with the Vitreoscilla hemoglobin geneen_US
dc.typeArticleen_US

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