Rhamnolipid production by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin gene.

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dc.authorscopusid6603935098
dc.authorscopusid6506575221
dc.contributor.authorKahraman H.
dc.contributor.authorErenler S.O.
dc.date.accessioned2024-08-04T19:59:30Z
dc.date.available2024-08-04T19:59:30Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe potential of Pseudomonas aeruginosa expressing the Vitreoscilla hemoglobin gene (vgb) for rhamnolipid production was studied. P. aeruginosa (NRRL B-771) and its transposon mediated vgb transferred recombinant strain, PaJC, were used in the research. The optimization of rhamnolipid production was carried out in the different conditions of cultivation (agitation rate, the composition of culture medium and temperature) in a time-course manner. The nutrient source, especially the carbon type, had a dramatic effect on rhamnolipid production. The PaJC strain and the wild type cells of P. aeruginosa started producing biosurfactant at the stationary phase and its concentration reached maximum at 24 h (838 mg/l(-1)) and at 72 h (751 mg l(-1)) of the incubation respectively. Rhamnolipid production was optimal in batch cultures when the temperature and agitation rate were controlled at 30 degrees C and 100 rpm. It reached 8373 mg l(-1) when the PaJC cells were grown in 1.0% glucose supplemented minimal media. Genetic engineering of biosurfactant producing strains with vgb may be an effective method to increase its production.en_US
dc.identifier.endpage217en_US
dc.identifier.issn0555-1099
dc.identifier.issue2en_US
dc.identifier.pmid22586915en_US
dc.identifier.scopus2-s2.0-84861662886en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage212en_US
dc.identifier.urihttps://hdl.handle.net/11616/90670
dc.identifier.volume48en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofPrikladnaia biokhimiia i mikrobiologiiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbacterial proteinen_US
dc.subjectglucoseen_US
dc.subjectglycolipiden_US
dc.subjecthemoproteinen_US
dc.subjectrhamnolipiden_US
dc.subjectsurfactanten_US
dc.subjectVgb protein, Bacteriaen_US
dc.subjectarticleen_US
dc.subjectbacterium transformationen_US
dc.subjectbiosynthesisen_US
dc.subjectfermentationen_US
dc.subjectgenetic engineeringen_US
dc.subjectgeneticsen_US
dc.subjectmetabolismen_US
dc.subjectmethodologyen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjecttemperatureen_US
dc.subjecttransposonen_US
dc.subjectVitreoscillaen_US
dc.subjectBacterial Proteinsen_US
dc.subjectDNA Transposable Elementsen_US
dc.subjectFermentationen_US
dc.subjectGenetic Engineeringen_US
dc.subjectGlucoseen_US
dc.subjectGlycolipidsen_US
dc.subjectHemeproteinsen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectSurface-Active Agentsen_US
dc.subjectTemperatureen_US
dc.subjectTransformation, Bacterialen_US
dc.subjectVitreoscillaen_US
dc.titleRhamnolipid production by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin gene.en_US
dc.typeArticleen_US

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