Investigation of EDTA-CarbaNP-direct test for the detection of metallo-β-lactamases in Pseudomonas aeruginosa

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dc.contributor.authorTutan, Hanife
dc.contributor.authorMazlumoglu, Bilge
dc.contributor.authorCizmeci, Zeynep
dc.contributor.authorCekin, Zuhal Kalayci
dc.contributor.authorComert, Fusun
dc.contributor.authorTanriverdi, Elif Seren
dc.contributor.authorAktas, Elif
dc.date.accessioned2026-04-04T13:35:09Z
dc.date.available2026-04-04T13:35:09Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractBackground: WHO has included carbapenem-resistant Pseudomonas aeruginosa (CR-Pa) in the high priority pathogens list. Ceftazidime-avibactam (CZA) is one of the limited treatment options in CR-Pa infections. Early detection of metallo-beta-lactamases (MBL) is important because CZA is not effective against MBL-producing isolates. Aim: To modify the CarbaNP-direct test (CNPdt) with EDTA and evaluate its use in MBL detection and prediction of CZA resistance CR-Pa isolates. Methods: 398 CR-Pa isolates from five centres in T & uuml;rkiye were included. Susceptibility tests were done using EUCAST criteria. The isolates were tested for blaVIM, blaIMP, blaNDM, blaOXA-48, blaKPC and blaGES genes using Biospeedy Carbapenem-Resistance qPCR kit and GES Antibiotic-Resistance kit (Bioeksen, T & uuml;rkiye). We modified CNPdt described by Pasteran et al. by adding a third tube with ethylenediaminetetraacetic acid (EDTA). The isolates with carbapenemase genes were subjected to CNPdt and EDTA-CNPdt. EDTA-CNPdt was considered positive if the tube without EDTA turned yellow while the tube with EDTA remained red and negative if both tubes turned yellow (Fig. 1). Results: Carbapenemase genes were detected in 55 (13.8 %) of the isolates. CNPdt was positive in 31 of the isolates. Of the 31 isolates, 23 were positive for EDTA-CNPdt. All of these 23 isolates were MBL producers and resistant to CZA. PCR, CNPdt, EDTA-CNPdt and CZA susceptibility test results are shown in Table 2. Conclusion: The positivity of CNPdt in CR-Pa is limited, but EDTA-CNPdt detected 100 % of MBL-producing isolates when CNPdt was positive. This test can be used for MBL detection and prediction of CZA resistance in CNPdt positive isolates.
dc.identifier.doi10.1016/j.diagmicrobio.2025.117056
dc.identifier.issn0732-8893
dc.identifier.issn1879-0070
dc.identifier.issue4
dc.identifier.orcid0000-0001-5293-2940
dc.identifier.orcid0000-0002-0449-0356
dc.identifier.pmid40795695
dc.identifier.scopus2-s2.0-105012922127
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.diagmicrobio.2025.117056
dc.identifier.urihttps://hdl.handle.net/11616/109656
dc.identifier.volume113
dc.identifier.wosWOS:001554452800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectCarbapenem resistance
dc.subjectCeftazidime/avibactam
dc.subjectMetallo-beta-lactamases
dc.subjectPseudomonas aeruginosa
dc.subjectCarbaNP-direct test
dc.subjectCarbapenemase
dc.titleInvestigation of EDTA-CarbaNP-direct test for the detection of metallo-β-lactamases in Pseudomonas aeruginosa
dc.typeArticle

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