Simultaneous quantification of acylated and desacylated ghrelin in biological fluids

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dc.authoridGeckil, Hikmet/0000-0003-0070-0691;
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.authorwosidKARATAS, Fikret/W-3340-2018
dc.contributor.authorAydin, Suleyman
dc.contributor.authorKaratas, Fikret
dc.contributor.authorGeckil, Hikmet
dc.date.accessioned2024-08-04T20:31:07Z
dc.date.available2024-08-04T20:31:07Z
dc.date.issued2008
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study reports simultaneous quantification of both acylated and desacylated forms of ghrelin in biological samples, utilizing a reverse-phase high-performance liquid chromatography (HPLC) system. The HPLC assay was also compared with RIA assays in use. Biological samples (serum, saliva, urine, milk) known for the presence of ghrelin were collected from a total of eight post-partum women and eight male volunteers. Analysis of ghrelin with HPLC was also validated for linearity, precision, detection limit and accuracy. An elution time of 6 min was observed for pure (commercial) desacylated human ghrelin and for the same form of the hormone from all body fluids studied. The elution time for acylated pure human ghrelin and that in body fluids, however, was around 16 min. The mean recovery rate was over 90% for both forms with no significant interference. The lowest detectable levels for acylated and desacylated ghrelin with the method used here were 11 (+/- 2) and 14 (+/- 3) pg mL(-1), respectively. Given its simplicity, accuracy, time and cost-effectiveness, the HPLC method described here for determination of two forms of ghrelin (active and inactive) might prove useful for certain diagnostic purposes. Copyright (C) 2008 John Wiley & Sons, Ltd.en_US
dc.identifier.doi10.1002/bmc.1065
dc.identifier.endpage1359en_US
dc.identifier.issn0269-3879
dc.identifier.issn1099-0801
dc.identifier.issue12en_US
dc.identifier.pmid18651597en_US
dc.identifier.scopus2-s2.0-59649118194en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1354en_US
dc.identifier.urihttps://doi.org/10.1002/bmc.1065
dc.identifier.urihttps://hdl.handle.net/11616/94747
dc.identifier.volume22en_US
dc.identifier.wosWOS:000261825400004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiomedical Chromatographyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectlipopeptidesen_US
dc.subjectghrelinen_US
dc.subjectdesacylated ghrelinen_US
dc.subjectacylated ghrelinen_US
dc.subjectn-octanoylationen_US
dc.titleSimultaneous quantification of acylated and desacylated ghrelin in biological fluidsen_US
dc.typeArticleen_US

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