Ratlarda deneysel periodontitis modelinde arjinin silikat inositol kompleksin periodontal dokulara etkisinin biyokimyasal, histopatolojik ve radyografik olarak incelenmesi
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2014
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İnönü Üniversitesi
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info:eu-repo/semantics/embargoedAccess
Özet
Arjinin silikat inositol (ASİ) kompleksi oluşturan arjininin ve silikonun; kemik mineral yoğunluğunu, kıkırdak, bağ ve damar dokularının kollajen miktarını arttırabileceği yönündeki çalışmaların sonuçlarına dayanılarak periodontal hastalıklarda konak modülasyonu amacıyla alternatif olabileceği düşünülmektedir. Bu araştırmanın amacı önceden ASİ kompleks diyet ile beslenen ratlarda periodontitis oluşturarak bu kompleksin periodontal dokulara etkisini biyokimyasal, immünolojik, histopatolojik ve radyolojik değerlendirmektir. Gereç ve Yöntem: Ağırlıkları ortalama 138 gr olan 8 haftalık dişi 52 sprague dawley rat 4 gruba ayrıldı. Gruplar; 1) Konrol Grubu (n=13): Standart rat diyetiyle deney süresince beslenen ve hiçbir işlemin uygulanmadığı grup. 2) Periodontitis Grubu (n=13): Standart rat diyetiyle 8 hafta süresince beslendikten sonra 11 günlük deneysel periodontitis oluşturulan grup. 3) ASİ I Grubu (n=13): Arjinin silikat inositol kompleksin 8 hafta boyunca 1,81 gr / kg-yem ölçeğinde standart yeme katılarak verildiği ve 8. haftanın sonunda 11 günlük deneysel periodontitis oluşturulan grup. 4) ASİ II Grubu (n=13): Arjinin silikat inositol kompleksin 8 hafta boyunca 3,62 gr/ kg-yem ölçeğinde standart yeme katılarak verildiği ve 8. haftanın sonunda 11 günlük deneysel periodontitis oluşturulan grup. On bir günlük deneysel periodontitis süresince tüm ratların beslenmesine standart yem ile devam edildi. On birinci günün sonunda tüm ratlar sakrifiye edilerek doku ve kan örnekleri alındı. Ratların sağ alt çenelerinden alınan dişeti dokularından western blot (WB) yöntemiyle interlökin 1 β (IL-1β), matriks metalloproteinaz-8 (MMP-8), reseptör aktivatör nükleer faktör kappa β (RANK), reseptör aktivatör nükleer faktör kappa β ligand (RANKL), osteoprotegerin (OPG), myeloperoksidaz (MPO) ve kan serumlarından alkalen fosfataz (ALP), kalsiyum (Ca), fosfor (P), C-reaktif protein (CRP) değerlendirilmeleri yapıldı. Mikro-komputerize tomografi verileri ile kemik yıkımı ölçümü ve kemik mineral dansitesi analizleri sağ alt çenelerden yapıldı. Histopatolojik incelemeler sol alt çenede yapıldı. Bulgular: C Reaktif protein, ASİ I ve II gruplarında periodontitis grubundan istatistiksel olarak anlamlı düzeyde düşük tespit edilmiştir (p<0.01). İnterlökin-1β ve MPO seviyeleri periodontitis grubunda ASİ'li gruplardan fazla, ASİ II grubunda ASİ I grubundan daha düşük tespit edilmiştir (p<0.05). Matriks metalloproteinaz-8, RANK, RANKL seviyeleri periodontitis grubunda ASİ'li gruplardan yüksek tespit edilmiştir (p<0.05). Osteoprotegerin seviyesi periodontitis grubunda ASİ'li gruplardan düşük, ASİ II grubunda ASİ I grubundan fazla tespit edilmiştir (p<0.05). Enflamatuvar hücre infiltrasyonu ASİ ile beslenen gruplarda periodontitis grubuna göre düşük tespit edilmiştir (p<0,01). ASİ'li grupların meziyal ve furkasyon bölgesindeki kemik yıkımı oranları periodontitis grubundan düşük tespit edilmiştir (p<0,01). Sonuç: Bu çalışmanın bulguları ASİ kompleks içeren diyet ile beslenen hayvanlarda deneysel periodontitis sonrası periodontal doku yıkımı ile ilişkili enzim/sitokin ve mediyatörlerin daha az olduğunu göstermiştir. Sonuçlar bu kompleksin konak modülasyonunda bir alternatif olabileceğini düşündürmektedir.
Introduction and Purpose: Arginine and silicate that compromise arginine silicate inositol (ASI) is considered to be alternative to host modulation in periodontal diseases based on the results of studies pointing out that it could increase bone mineral density and the amount of collagen cartilage, connective and vessels tissue. The purpose of this study was to experimentally induce periodontitis in rats fed previously with diets of arginine silicate inositol (ASI) complex and examine the effect of this complex by biochemical, immunological, histopathological, and radiological evaluation. Materials and Methods: Fifty-two 8-week-old female Sprague–Dawley rats, weighing 138 g, were equally divided into 4 groups. Groups; 1) The control group included animals fed a standard rat diet with no operation performed during the experiment. 2) The periodontitis group, 3) ASI I, and 4) ASI II groups were subjected to experimental periodontitis induction for 11 days after being fed a standard rat diet alone and that containing 1.81 g/kg and 3.62 g/kg ASI complex, respectively, for 8 weeks. Throughout the 11-day duration of experimental periodontitis induction, all rats were fed standard feed. At the end of the 11th day, all rats were killed and their tissue and blood samples were collected. Interleukin (IL)-1β, matrix metalloproteinase (MMP)-8, receptor activator of nuclear factor kappa -β (RANK), receptor activator of nuclear factor kappa-β ligand (RANKL), osteoprotegerin (OPG), myeloperoxidase (MPO) levels were evaluated using western blot analysis using the gum tissue obtained from the right mandible, and alkaline phosphatase (ALP), calcium (Ca), phosphor (P), C-reactive protein (CRP) levels were evaluated using blood samples. Bone resorption and bone mineral density were evaluated using microcomputed tomography of the right mandible. Tissues collected from the left mandible were subjected to histopathological evaluation. Results: C- reactive protein levels have been found to be statistically significantly lower in the two ASI groups than in the periodontitis group (p<0.01). Interleukin -1β and MPO levels were higher in the periodontitis group than in the ASI groups, with the ASI II group showing lower levels than the ASI group (p<0.05). Matrix metalloproteinase -8, RANK, and RANKL levels were higher in periodontitis groups than in the ASI groups (p<0.05). Osteoprotegerin level was lower in periodontitis groups than in the ASI groups, with the ASI II group showing higher level than the ASI I group (p<0,05). Inflammatory cell infiltration and bone resorption rates in the mesial and furcation regions were lower in the ASI groups than in the periodontitis group (p<0.01). Conclusion: The findings of this study shows that enzyme / cytokines and mediators associated with periodontal tissue destruction is less in animals fed a diet containing ASI complex after experimental periodontitis. Results make experts think that this complex would be an alternative in host modulation.
Introduction and Purpose: Arginine and silicate that compromise arginine silicate inositol (ASI) is considered to be alternative to host modulation in periodontal diseases based on the results of studies pointing out that it could increase bone mineral density and the amount of collagen cartilage, connective and vessels tissue. The purpose of this study was to experimentally induce periodontitis in rats fed previously with diets of arginine silicate inositol (ASI) complex and examine the effect of this complex by biochemical, immunological, histopathological, and radiological evaluation. Materials and Methods: Fifty-two 8-week-old female Sprague–Dawley rats, weighing 138 g, were equally divided into 4 groups. Groups; 1) The control group included animals fed a standard rat diet with no operation performed during the experiment. 2) The periodontitis group, 3) ASI I, and 4) ASI II groups were subjected to experimental periodontitis induction for 11 days after being fed a standard rat diet alone and that containing 1.81 g/kg and 3.62 g/kg ASI complex, respectively, for 8 weeks. Throughout the 11-day duration of experimental periodontitis induction, all rats were fed standard feed. At the end of the 11th day, all rats were killed and their tissue and blood samples were collected. Interleukin (IL)-1β, matrix metalloproteinase (MMP)-8, receptor activator of nuclear factor kappa -β (RANK), receptor activator of nuclear factor kappa-β ligand (RANKL), osteoprotegerin (OPG), myeloperoxidase (MPO) levels were evaluated using western blot analysis using the gum tissue obtained from the right mandible, and alkaline phosphatase (ALP), calcium (Ca), phosphor (P), C-reactive protein (CRP) levels were evaluated using blood samples. Bone resorption and bone mineral density were evaluated using microcomputed tomography of the right mandible. Tissues collected from the left mandible were subjected to histopathological evaluation. Results: C- reactive protein levels have been found to be statistically significantly lower in the two ASI groups than in the periodontitis group (p<0.01). Interleukin -1β and MPO levels were higher in the periodontitis group than in the ASI groups, with the ASI II group showing lower levels than the ASI group (p<0.05). Matrix metalloproteinase -8, RANK, and RANKL levels were higher in periodontitis groups than in the ASI groups (p<0.05). Osteoprotegerin level was lower in periodontitis groups than in the ASI groups, with the ASI II group showing higher level than the ASI I group (p<0,05). Inflammatory cell infiltration and bone resorption rates in the mesial and furcation regions were lower in the ASI groups than in the periodontitis group (p<0.01). Conclusion: The findings of this study shows that enzyme / cytokines and mediators associated with periodontal tissue destruction is less in animals fed a diet containing ASI complex after experimental periodontitis. Results make experts think that this complex would be an alternative in host modulation.
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05.09.2017 tarihine kadar kullanımı yazar tarafından kısıtlanmıştır.
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Dündar, S. (2014). Ratlarda deneysel periodontitis modelinde arjinin silikat inositol kompleksin periodontal dokulara etkisinin biyokimyasal, histopatolojik ve radyografik olarak incelenmesi. İnönü Üniversitesi Sağlık Bilimleri Dergisi. 1-156 ss.
