Molecular Cloning and Sequence Analysis of the its Region of Nuclear Ribosomal DNA for Species Identification in Dodders (Cuscuta; Convolvulaceae)

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dc.authorwosidSipahioglu, Hikmet Murat/AAA-6085-2020
dc.authorwosidUsta, Mustafa/CAG-7521-2022
dc.contributor.authorKeskin, Fatma
dc.contributor.authorKaya, Ilhan
dc.contributor.authorUsta, Mustafa
dc.contributor.authorDemir, Ibrahim
dc.contributor.authorSipahioglu, Hikmet Murat
dc.contributor.authorNemli, Yildiz
dc.date.accessioned2024-08-04T20:44:06Z
dc.date.available2024-08-04T20:44:06Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractDodder (Cuscuta sp.) is an obligate parasitic plant that is very difficult to control. In plants the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal DNA (nrDNA) has been considered one of the most important sequences for phylogenetic analysis. Here we report the analysis of nrDNA's ITS sequences as an efficient tool to study the phylogeny of dodders collected from various provinces of Eastern Anatolia (Turkey). Genomic DNA of six dodder samples belonging to 4 distinct species was extracted from body tissue samples. The sequences of 18S rRNA, ITS-1, 5.8S rRNA, ITS-2 and 26S rRNA regions of 4 Cuscuta species were determined by molecular cloning and sequencing. The identity of cloned fragments was compared to determine sequence identity using NCBI database. Bootstrap analysis of nrDNA of C. approximate, C. lupuliformis, C. campestris and C. babylonica indicated high sequence identity with similar sequences belonging to different geographical origins of the world retrieved from NCBI database. Our results clearly showed that the most stable secondary structure derived from the sequences obtained by universal ITS4 and ITS5 primers is very efficient tool for identification of Cuscuta species when used in combination with phylogenetic analysis. (C) 2017 Friends Science Publishersen_US
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113O418]en_US
dc.description.sponsorshipThe authors wish to thank to TUBITAK (The Scientific and Technological Research Council of Turkey, Project no: 113O418) for the financial support. This research paper was produced from Fatma Keskin's M.Sc. thesis research.en_US
dc.identifier.doi10.17957/IJAB/15.0442
dc.identifier.endpage1451en_US
dc.identifier.issn1560-8530
dc.identifier.issn1814-9596
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85034030972en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1447en_US
dc.identifier.urihttps://doi.org/10.17957/IJAB/15.0442
dc.identifier.urihttps://hdl.handle.net/11616/98026
dc.identifier.volume19en_US
dc.identifier.wosWOS:000416107100021en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFriends Science Publen_US
dc.relation.ispartofInternational Journal of Agriculture and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectClassificationen_US
dc.subjectCuscuta sppen_US
dc.subjectEast anatoliaen_US
dc.subjectIdentificationen_US
dc.subjectMolecular phylogenyen_US
dc.titleMolecular Cloning and Sequence Analysis of the its Region of Nuclear Ribosomal DNA for Species Identification in Dodders (Cuscuta; Convolvulaceae)en_US
dc.typeArticleen_US

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