Amifostine enhances the antioxidant and hepatoprotective effects of UW and HTK preservation solutions

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dc.authoridDagdeviren, Attila/0000-0001-8990-8282
dc.authoridOksuz, Ergun/0000-0002-5723-5965
dc.authoridAkbulut, Sami/0000-0002-6864-7711
dc.authoridSevmis, Sinasi/0000-0001-8728-5472
dc.authoridBAYRAKTAR, NILUFER/0000-0002-7886-3688
dc.authorwosidDagdeviren, Attila/P-2877-2014
dc.authorwosidOksuz, Ergun/K-8238-2012
dc.authorwosidAkbulut, Sami/L-9568-2014
dc.authorwosidDağdeviren, Attila/AES-7155-2022
dc.authorwosidSevmis, Sinasi/JOZ-7660-2023
dc.authorwosidBAYRAKTAR, NILUFER/Y-8758-2018
dc.contributor.authorAkbulut, Sami
dc.contributor.authorSevmis, Sinasi
dc.contributor.authorKarakayali, Hamdi
dc.contributor.authorBayraktar, Nilufer
dc.contributor.authorUnlukaplan, Muge
dc.contributor.authorOksuz, Ergun
dc.contributor.authorDagdeviren, Atilla
dc.date.accessioned2024-08-04T20:39:54Z
dc.date.available2024-08-04T20:39:54Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAIM: To investigate whether amifostine contributes to the antioxidant and cytoprotective effects of histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin (UW) preservation solutions. METHODS: Forty-eight Sprague Dawley male rats were equally divided into six groups: (1) ringer Lactate (RL) group; (2) RL + amifostine (RL + A) group; (3) HTK group; (4) HTK + A group; (5) UW group; and (6) UW + A group. Rats in the RL + A, HTK + A and UW + A groups were administered amifostine intraperitoneally at a dose of 200 mg/kg prior to laparotomy. The RL group was perfused with RL into the portal vein. The RL + A group were perfused with RL into the portal vein after amifostine administration. The HTK group received an HTK perfusion while the HTK + A group received an HTK perfusion after administration of amifostine. The UW group received a perfusion of UW, while the UW + A group received a UW perfusion after amifostine administration. Liver biopsy was performed to investigate histopathological, immunochemical [transferase mediated dUTP nick end labeling (TUNEL), inducible nitric oxide syntetase (iNOS)] and ultrastructural alterations. Biochemical alterations were determined by examining levels of alanine aminotransferase, alkaline phosphatase and nitric oxide in the perfusion fluid. RESULTS: Pathological sinusoidal dilatation and centrilobular hydropic alteration were significantly lower in the groups that received amifostine prior to preservation solution perfusion. Although the best results were obtained in the UW + A group, we did not observe a statistically significant difference between the UW + A and HTK + A groups. iNOS grades were significantly lower in the amifostine groups 12 h after treatment. When the amifostine groups were compared against each other, the iNOS grades obtained from the UW + A and HTK + A groups were similar while the RL + A group had a much poorer score. TUNEL assays demonstrated a lower apoptosis ratio in the amifostine groups than in the non-amifostine groups 12 h after treatment. No statistically significant difference was observed between the UW + A and HTK + A groups for apoptosis. Cellular ultrastructure was best preserved in the UW + A and HTK + A groups. CONCLUSION: Here, we show that preoperative administration of a single dose of amifostine is sufficient to minimize the preservation damage in hepatic cells. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.en_US
dc.identifier.doi10.3748/wjg.v20.i34.12292
dc.identifier.endpage12300en_US
dc.identifier.issn1007-9327
dc.identifier.issn2219-2840
dc.identifier.issue34en_US
dc.identifier.pmid25232264en_US
dc.identifier.scopus2-s2.0-84909609683en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage12292en_US
dc.identifier.urihttps://doi.org/10.3748/wjg.v20.i34.12292
dc.identifier.urihttps://hdl.handle.net/11616/96588
dc.identifier.volume20en_US
dc.identifier.wosWOS:000341719100038en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBaishideng Publishing Group Incen_US
dc.relation.ispartofWorld Journal of Gastroenterologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLiveren_US
dc.subjectTransplantationen_US
dc.subjectPreservation solutionsen_US
dc.subjectHistidine-tryptophan-ketoglutarateen_US
dc.subjectUniversity of Wisconsinen_US
dc.subjectAntioxidanten_US
dc.subjectAmifostineen_US
dc.titleAmifostine enhances the antioxidant and hepatoprotective effects of UW and HTK preservation solutionsen_US
dc.typeArticleen_US

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