Effect of bilirubin in ischemia reperfusion injury on rat small intestine
Küçük Resim Yok
Dosyalar
Tarih
2001
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Journal of Pediatric Surgery
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: The aim of this study was to determine the effects
of bilirubin in experimental small intestinal ischemia/reperfusion
(I/R) injury in rats.
Methods: Thirty rats were divided into 5 groups (n 5 6). In
group S, saline and in group B, bilirubin, 20 mg/kg were
infused via the jugular vein without an additional procedure.
In groups S-IR, saline, B1-IR and B2-IR, 10 and 20 mg/kg/h of
bilirubin were infused for 2 hours, respectively. In these
groups, an I/R procedure was done after infusions by occluding
the superior mesenteric artery for 45 minutes followed by
1 hour of reperfusion. After reperfusion, the small intestines
were resected for histopathologic and malondialdehyde
(MDA) assessments. Mucosal lesions were scored between 0
and 5. Malondialdehyde levels and histopathologic grades
were analyzed statistically.
Results: Mucosal injury was severe in S-IR (grade 4 to 5),
mild in B1-IR (grade 0 to 3) and none in B2-IR group (grade 0).
Grades of group S-IR were higher than those of B1-IR and
B2-IR statistically (P ,.05). Tissue MDA levels of the S-IR
group were significantly higher than those of B1-IR and B2-IR
groups (U 5 36, P , .05). Bilirubin levels correlated inversely
with MDA levels (r 5 20.94).
Conclusions: Bilirubin effectively prevents intestinal I/R injury
in rat. This observation is consistent with the hypotheses
regarding bilirubin as an antioxidant, having a role in the
body defense.
Açıklama
Anahtar Kelimeler
Bilirubin, Ischemia/reperfusion injury, Intestine
Kaynak
Journal of Pediatric Surgery
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
12
Künye
Ceran Özcan, C. Sönmez, K. Türkyılmaz, Z. Demiroğulları, B. Dursun, A. Düzgün, E. Başaklar, A. C. Kale, N. (2001). Effect of bilirubin in ischemia reperfusion injury on rat small intestine. Journal of Pediatric Surgery, 36(12), 1764–1767.
