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    The beneficial effects of 18 glycyrrhetinic acid following oxidative and neuronal damage in brain tissue caused by global cerebral ischemia reperfusion in a C57BL J6 mouse model
    (Neurol Sci, 2014) Öztanır, Mustafa Namık; Çiftçi, Osman; Çetin, Aslı; Durak, Mehmet Akif; Başak, Neşe; Akyuva, Yener
    This study investigated the effects of 18bglycyrrhetinic acid (GA) on neuronal damage in brain tissue caused by global cerebral ischemia/reperfusion (I/R) in C57BL/J6 mice. All subjects (n = 40) were equally divided into four groups: (1) sham-operated (SH), (2) I/R, (3) GA, and (4) GA?I/R. The SH group was used as a control. In the I/R group, the bilateral carotid arteries were clipped for 15 min, and the mice were treated with the vehicle for 10 days. In the GA group, mice were given GA (100 mg/ kg) for 10 days following a median incision without carotid occlusion. In the GA?I/R group, the I/R model was applied to the mice exactly as in the I/R group, and they were then treated with the same dose of GA for 10 days. Cerebral I/R significantly induced oxidative stress via an increase in lipid peroxidaitons and a decrease in elements of the antioxidant defense systems. However, GA treatment was protective against the oxidative effects of I/R by inducing significant increases in antioxidant defense systems and a significant decrease of lipid peroxidations. Additionally, cerebral I/R increased the incidence of histopathological damage and apoptosis in brain tissue, but these neurodegenerative effects were eliminated by GA treatment. Therefore, the current study demonstrated that GA treatment effectively prevents oxidative and histological damage in the brain caused by global I/R. In this context, GA may be useful for the attenuation of the negative effects of global cerebral I/R and, in the future, it may be a viable and safe alternative treatment for ischemic stroke in humans.
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    Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
    (BMC, CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND, 2018) Melekoğlu, Rauf; Çiftçi, Osman; Çetin, Aslı; Başak, Neşe
    Background: In recent years, cancer rates have been rising among reproductive-age women. Thus, chemotherapy exposure has become an important cause of premature ovarian failure (POF). There has been growing interest regarding the preservation and restoration of ovarian function before and after oncological treatment because of the reproductive risk of chemotherapeutics and improved long-term survival of cancer patients. In this study, we sought to analyze the effects of curcumin (CRC) and capsaicin (CPS) on cyclophosphamide-induced POF in a rat model. Methods: POF in rats was induced by intraperitoneal injection of 200 mg/kg cyclophosphamide on day 1 and then 8 mg/kg/day for the following 14 days. After 14 days of cyclophosphamide administration, rats were randomly divided into three groups as follows (n = 10/group): POF, POF + CRC (100 mg/kg/day), and POF + CPS (0.5 mg/kg/day) to determine the effects of CRC and CPS on the cyclophosphamide-induced POF rat model. Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after the CRC and CPS treatments. Results: Malonaldehyde levels were significantly reduced, and glutathione levels and superoxide dismutase activity were significantly increased, in ovarian tissues in the POF + CRC and POF + CPS groups compared with the POF group. In the POF group, we observed hemorrhage and prominent mononuclear cell infiltration beneath the germinative epithelium, vascular congestion in ovarian stroma, hemorrhage around the corpus luteum, and atresia in ovarian follicles. This histopathological damage was significantly improved by treatment with CRC and CPS. There was a significant reduction in serum follicle-stimulating hormone and luteinizing hormone levels in rats treated with CRC and CPS compared with the POF group. Moreover, the levels of estradiol and anti-mullerian hormone in rats treated with CRC and CPS were significantly increased compared with the control group. Conclusions: In conclusion, CRC and CPS treatment of rats with cyclophosphamide-induced POF had a beneficial effect on reducing ovarian damage by improving tissue oxidative stress marker levels, ovarian reserve marker levels, and histopathological parameters. The significant improvements in ovarian tissue histopathological damage and hormonal levels detected in this study indicate that treatment with CRC or CPS might be a conservative treatment approach for cyclophosphamide-induced POF.
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    Beneficial effects of glucan against cisplatin side effects on the nervous system in rats 1
    (Acta cir brasl, 2016) Kaya, Kürşat; Çiftçi, Osman; Çetin, Aslı; Tecellioğlu, Mehmet; Başak, Neşe
    PURPOSE: To investigate the protective effect of Bg on cisplatin (CP)-induced neurotoxicity in rats. METHODS: Twenty eight rats were randomly distributed into four groups. The first group was kept as a control. In the second group, CP was given at the single dose of 7 mg/kg intraperitoneally. In the third group, βg was orally administered at the dose of 50 mg/kg/day for 14 days. In the fourth group, CP and βg were given together at the same doses. RESULTS: CP treatment caused significant oxidative damage via induction of lipid peroxidation and reductions antioxidant defense system potency in the brain tissue. In addition, histopathological damage increased with CP treatment. On the other hand, βg treatment largely prevented oxidative and histopathological negative effects of CP. CONCLUSIONS: Cisplatin has severe neurotoxic effects in rats and βg supplementation has significant beneficial effects against CP toxicity depending on its antioxidant properties. Thus, it appears that βg might be useful against CP toxicity in patients with cancer in terms of nervous system.
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    Beneficial effects of hesperidin following cis diamminedichloroplatinum induced damage in heart of rats
    (Niger J Clin Pract., 2016) Oğuztürk, Hakan; Çiftçi, Osman; Çetin, Aslı; Kaya, Kürşat; Dişli, Om; Turtay, Muhammet Gökhan; Gürbüz, Şükrü; Başak, Neşe
    Background: Increased oxidative stress and histopathological damage have been implicated in the cardiotoxicity that limits the clinical therapy of cisplatin (CP) as an anti-cancer drug. Objectives: This study aimed to investigate the protective effect of hesperidin (HP) against CP-induced cardiotoxicity in rats. Materials and Methods: Rats were divided into four groups (n = 7/group), and the first group served as the control group. Animals in Group CP and Group CP + HP received a single dose of CP (CP - 7 mg/kg); animals in Group HP and Group CP + HP received 50 mg/kg/day HP with gavage for 14 days. At the end of day 14, cardiac tissue samples were histologically and biochemically examined. Results: In this experimental study, thiobarbituric acid reactive substances levels in the cardiac tissue were significantly higher in the CP group, whereas glutathione (GSH), superoxide dismutase (SOD), and CAT levels were significantly lower in this group. On the other hand, GSH and SOD levels in the CP + HP group were similar to the control group. There was no significant difference in cardiac CAT levels between Group CP and Group CP + HP. Conclusion: Hesperetin treatment leads to a decrease in oxidative stress, and associated histological damage. The findings of the current study suggest that HP has a protective effect against CP‑induced cardiotoxicity.
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    The beneficial effects of Montelukast against 2 3 7 8 tetrachlorodibenzo p dioxin toxicity in female reproductive system in rats
    (Acta Cir Bras., 2016) Melekoğlu, Rauf; Çiftçi, Osman; Çetin, Aslı; Başak, Neşe; Çelik, Ebru
    PURPOSE: To determine the toxic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on reproductive system and the beneficial effects of Montelukast (ML) with histological and biochemical analysis. METHODS: Rats were randomly divided into four equal groups (control, TCDD, ML and TCDD+ML). Tissue samples were collected on day 60 and oxidative status and histological alterations were analyzed. RESULTS: The results showed a significant increase in oxidative and histological damage on uterine and ovarian tissues. Otherwise, the oxidative and histological damages caused by TCDD were prevented with ML treatment. CONCLUSION: The toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on female reproductive system were reversed with Montelukast treatment. Therefore, we claimed that ML treatment might be useful for TCDD toxicity.
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    The beneficial effects of nerolidol and hesperidin on surgically induced endometriosis in a rat model
    (Gynecologıcal endocrınology, 2018) Melekoğlu, Rauf; Çiftlikci, Osman; Eraslan, Sevil; Çetin, Aslı; Basak, Nese
    The objective of this article is to analyze the effects of nerolidol and hesperidin treatment on surgically induced endometriosis in a rat model. Endometriosis was induced in 24 healthy adult female Wistar albino rats via homologous uterine horn transplantation. Three operations were performed on each rat. After the second operation, the rats were randomized into control, nerolidol, and hesperidin treatment groups, and medications were administered for 2 weeks. The effects of the drugs on the endometriotic foci were evaluated after the third operation. Compared with the endometriosis control group, the average volume of the lesions was significantly lower in rats treated with hesperidin and nerolidol. Malondialdehyde levels were significantly reduced in the nerolidol-treated group, and glutathione levels and superoxide dismutase activity were significantly elevated in the endometriotic foci of both the hesperidin- and nerolidol-treated groups compared with the endometriosis group. Hesperidin and nerolidol treatment also improved histological parameters, such as hemorrhage, vascular congestion, necrosis, and inflammatory cell infiltration in the endometriotic foci. The results of this study demonstrated that treatment with the potent antioxidants nerolidol and hesperidin caused a significant regression of surgically induced endometriotic foci in rats.
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    DENEYSEL DİYABETİN SIÇAN KALP DOKUSUNDA MEYDANA GETİRDİĞİ HİSTOLOJİK DEĞİŞİKLİKLER ÜZERİNE AMİNOGUANİDİNİN İYİLEŞTİRİCİ ETKİLERİ
    (2013) Orman, Doğan; VARDI, Nigar; Çetin, Aslı
    Öz Bu çalışma,Streptozotosin iledeneysel diyabet oluşturulan sıçanlarda kalp dokusunda meydana gelen histolojik değişiklikler üzerine aminoguanidininolasıiyileştirici etkilerinin gösterilmesi amacıyla planlandı. Çalışmada 32 adet Sprague Dawley erkek sıçan kullanıldı. Deneyde kullanılan sıçanlar her bir grupta 8 adet olacak şekilde 4 gruba ayrıldı. Gruplar sırası ile Kontrol, Aminoguanidin, Diyabet ve Diyabet + Aminoguanidin olarak belirlendi. Deneysel diyabet, tek doz Streptozotosin’ in intraperitoneal uygulanması ile oluşturuldu. Hematoksilen-Eozin, Masson Trikrom veToluidin Blue ile oyanan kesitler, Leica DFC 280 ışık mikroskobu veLeica Q Win Plus analiz sistemi kullanılarak incelendi.
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    Deneysel diyabetin sıçan kalp dokusunda meydana getirdiği histolojik değişiklikler üzerine aminoguanidinin iyileştirici etkileri
    (2013) Çetin, Aslı; Vardı, Nigar; Orman, Doğan
    Bu çalışma,Streptozotosin iledeneysel diyabet oluşturulan sıçanlarda kalp dokusunda meydana gelen histolojik değişiklikler üzerine aminoguanidininolasıiyileştirici etkilerinin gösterilmesi amacıyla planlandı. Çalışmada 32 adet Sprague Dawley erkek sıçan kullanıldı. Deneyde kullanılan sıçanlar her bir grupta 8 adet olacak şekilde 4 gruba ayrıldı. Gruplar sırası ile Kontrol, Aminoguanidin, Diyabet ve Diyabet + Aminoguanidin olarak belirlendi. Deneysel diyabet, tek doz Streptozotosin’ in intraperitoneal uygulanması ile oluşturuldu. Hematoksilen-Eozin, Masson Trikrom veToluidin Blue ile oyanan kesitler, Leica DFC 280 ışık mikroskobu veLeica Q Win Plus analiz sistemi kullanılarak incelendi.
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    Hesperidin a citrus flavonoid has the ameliorative effects against experimental autoimmune encephalomyelitis EAE in a C57BL J6 mouse model
    (Neurochemical Research, 2015) Çiftçi, Osman; Özcan, Abdulcemal; Kamışlı, Özden; Çetin, Aslı; Başak, Neşe; Bilal, Aytaç
    The aim of this study was determined the effects of Hesperidin (HP) on neuronal damage in brain tissue caused by Experimental allergic encephalomyelitis (EAE), an established model of multiple sclerosis in C57BL/J6 mice. To explore 40 mice were equally divided into four groups: (1) Control, (2) EAE, (3) HP, and (4) HP ? EAE. 14 days after induction of EAE with MOG35- 55 and pertussis toxin, the mice treated with HP at the doses of 50 mg/kg/day for 7 days subcutaneously. To our results HP treatment prevents the oxidative stress caused by EAE via a decrease in lipid peroxidations and increase in elements of the antioxidant defense systems in brain tissue. Also, EAE elevate the IL-17, express the pro-in- flammatory cytokines, and caspase-3-like immunreactivity, show apoptosis, staining in EAE mice brain and increased the incidence of histopathological damage. However, immonohistochemical and histological changes were reversed with HP. Moreover, elevated TNF-a and IL-1b levels, a result of EAE, were decreased in serum and neurological deficits as clinical signs were reversed with HP treatment in EAE mice, given HP. In conclusion, HP treatment effectively prevents oxidative, immunological and histological damage in the brain caused by EAE. It was thought that the beneficial effects of HP are likely a result of its strong antioxidant and anti-inflammatory properties.
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    Hesperidin a citrus flavonoid has the ameliorative effects against experimental autoimmune encephalomyelitis EAE in a C57BL J6 mouse model
    (Neurochem Res, 2015) Çiftçi, Osman; Özcan, Cemal; Kamışlı, Özden; Çetin, Aslı; Başak, Neşe; Aytaç, Bilal
    The aim of this study was determined the effects of Hesperidin (HP) on neuronal damage in brain tissue caused by Experimental allergic encephalomyelitis (EAE), an established model of multiple sclerosis in C57BL/J6 mice. To explore 40 mice were equally divided into four groups: (1) Control, (2) EAE, (3) HP, and (4) HP ? EAE. 14 days after induction of EAE with MOG35- 55 and pertussis toxin, the mice treated with HP at the doses of 50 mg/kg/day for 7 days subcutaneously. To our results HP treatment prevents the oxidative stress caused by EAE via a decrease in lipid peroxidations and increase in elements of the antioxidant defense systems in brain tissue. Also, EAE elevate the IL-17, express the pro-in- flammatory cytokines, and caspase-3-like immunreactivity, show apoptosis, staining in EAE mice brain and increased the incidence of histopathological damage. However, immonohistochemical and histological changes were reversed with HP. Moreover, elevated TNF-a and IL-1b levels, a result of EAE, were decreased in serum and neurological deficits as clinical signs were reversed with HP treatment in EAE mice, given HP. In conclusion, HP treatment effectively prevents oxidative, immunological and histological damage in the brain caused by EAE. It was thought that the beneficial effects of HP are likely a result of its strong antioxidant and anti-inflammatory properties.
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    Hesperidin protects brain and sciatic nerve tissues against cisplatin induced oxidative histological and electromyographical side effects in rats
    (Toxicology and Industrial Health, 2015) Kamışlı, Suat; Çiftçi, Osman; Kaya, Kürşat; Çetin, Aslı; Kamışlı, Özden; Özcan, Abdulcemal
    In the present study, the beneficial effect of hesperidin (HP), a citrus flavonoid, on cisplatin (CP)-induced neurotoxicity was investigated. A total of 28 rats were equally divided into four groups; the first group was kept as control. In the second and third groups, CP and HP were given at the doses of 7 and 50 mg/kg/day, respectively. In the fourth group, CP and HP were given together at the same doses. The results indicated that although CP caused significant induction of lipid peroxidations and reduction in the antioxidant defense system potency in the brain and sciatic nerve, HP prevented these effects of CP. Besides, CP led to histopathological damage, mainly apoptosis, as well as electromyographical (EMG) changes in sciatic nerve. On the other hand, HP treatment reversed histopathological and EMG effects of CP. In conclusion, CP had severe dose-limiting neurotoxic effects and these effects of CP can be prevented by HP treatment. Thus, it appears that coadministration of HP with CP may be a useful approach to attenuate the negative effects of CP on the nervous system.
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    Hesperidin protects testicular and spermatological damages induced by cisplatin in rats
    (Andrologia, 2015) Kaya, Kürşat; Çiftçi, Osman; Çetin, Aslı; Doğan, Halef; Başak, Neşe
    The clinic usage of cisplatin, an anticancer drug, is limited due to it has many side effects in many systems and organs. In this context, it was aimed to investigate the protective effect of hesperidin, a citrus flavonoid, on testicular and spermatological damages induced by cisplatin in rats. The rats were randomly divided into four groups. The first group was kept as a control. In the second groups, cisplatin was given at the single dose of 7 mg kg(-1) intraperitoneally. In the third group, hesperidin was orally administered at the dose of 50 mg/kg day(-1) for 14 days. In the fourth group, cisplatin and hesperidin were given together at the same doses. Cisplatin treatment caused significant reductions enzymatic (SOD, CAT and GPx) and nonenzymatic (GSH) antioxidants and significant induction level of TBARS. In addition, cisplatin treatment caused decreased sperm motility, epididymal sperm concentration, increased abnormal sperm rate and histopathological damage. In contrast, hesperidin treatment significantly attenuated the harmful effects. In conclusion, this study clearly demonstrated that hesperidin has protective effects on cisplatin-induced reproductive system toxicity depending on its antioxidant properties. Thus, it is thought that hesperidin may be useful against cisplatin toxicity in patients with cancer in terms of reproductive system.
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    Histopathological ultrastructural and apoptotic changes in diabetic rat placenta
    (Balkan Medical Journal, 2015) Gül, Mehmet; Bostancıeri, Nuray; Çetin, Aslı; Kepekçi, Remziye Aysun; Şimşek, Ömer Yavuz; Kayhan, Başak; Turhan, Uğur; Otlu, Ali
    The exchange of substances between mother and fetus via the placenta plays a vital role during development. A number of developmental disorders in the fetus and placenta are observed during diabetic pregnancies. Diabetes, together with placental apoptosis, can lead to developmental and functional disorders. Aims: Histological, ultrastructural and apoptotic changes were investigated in the placenta of streptozotocin (STZ) induced diabetic rats. Study Design: Animal experimentation. Methods: In this study, a total of 12 female Wistar Albino rats (control (n=6) and diabetic (n=6)) were used. Rats in the diabetic group, following the administration of a single dose of STZ, showed blood glucose levels higher than 200 mg/dL after 72 hours. When pregnancy was detected after the rats were bred, two pieces of placenta and the fetuses were collected on the 20th day of pregnancy by cesarean incision under ketamine/ xylazine anesthesia from in four rats from the control and diabetic groups. Placenta tissues were processed for light microscopy and transmission electron microscopy (TEM). Hematoxylin-eosin (HE) and periodic acid Schiff-diastase (PAS-D) staining for light microscopic and caspase-3 staining for immunohistochemical investigations were performed for each placenta. Electron microscopy was performed on thin sections contrasted with uranyl acetate and lead nitrate. Results: Weight gain in the placenta and fetuses of diabetic rats and thinning of the decidual layer, thickening of the hemal membrane, apoptotic bodies, congestion in intervillous spaces, increased PAS-D staining in decidual cells and caspase-3 immunoreactivity were observed in the diabetic group. After the ultrastructural examination, the apoptotic appearance of the nuclei of trophoblastic cells, edema and intracytoplasmic vacuolization, glycogen accumulation, dilation of the endoplasmic reticulum and myelin figures were observed. In addition, capillary basement membrane thickening, capillary endothelial cells chromatin condensation in the nucleus and corrugation of the nucleus were found. Conclusion: Diabetes causes histomorphometric, ultrastructural and apoptotic changes in rat placenta.
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    Histopathological, Ultrastructural and Apoptotic Changes in Diabetic Rat Placenta
    (GALENOS YAYINCILIK, 2015-06) Gül, Mehmet; Bayat, Nuray; Çetin, Aslı; Kepekci, Remziye Aysun; Şimşek, Yavuz; Kayhan, Başak; Turhan, Uğur; Otlu, Ali
    Background: The exchange of substances between mother and fetus via the placenta plays a vital role during development. A number of developmental disorders in the fetus and placenta are observed during diabetic pregnancies. Diabetes, together with placental apoptosis, can lead to developmental and functional disorders. Aims: Histological, ultrastructural and apoptotic changes were investigated in the placenta of streptozotocin (STZ) induced diabetic rats. Study Design: Animal experimentation. Methods: In this study, a total of 12 female Wistar Albino rats (control (n=6) and diabetic (n=6)) were used. Rats in the diabetic group, following the administration of a single dose of STZ, showed blood glucose levels higher than 200 mg/dL after 72 hours. When pregnancy was detected after the rats were bred, two pieces of placenta and the fetuses were collected on the 20th day of pregnancy by cesarean incision under ketamine/xylazine anesthesia from in four rats from the control and diabetic groups. Placenta tissues were processed for light microscopy and transmission electron microscopy (TEM). Hematoxylin-eosin (HE) and periodic acid Schiff-diastase (PAS-D) staining for light microscopic and caspase-3 staining for immunohistochemical investigations were performed for each placenta. Electron microscopy was performed on thin sections contrasted with uranyl acetate and lead nitrate. Results: Weight gain in the placenta and fetuses of diabetic rats and thinning of the decidual layer, thickening of the hemal membrane, apoptotic bodies, congestion in intervillous spaces, increased PAS-D staining in decidual cells and caspase-3 immunoreactivity were observed in the diabetic group. After the ultrastructural examination, the apoptotic appearance of the nuclei of trophoblastic cells, edema and intracytoplasmic vacuolization, glycogen accumulation, dilation of the endoplasmic reticulum and myelin figures were observed. In addition, capillary basement membrane thickening, capillary endothelial cells chromatin condensation in the nucleus and corrugation of the nucleus were found. Conclusion: Diabetes causes histomorphometric, ultrastructural and apoptotic changes in rat placenta
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    Karaciğer ve Pankreas Gelişimi
    (2017) Eşrefoğlu, Mukaddes; Taşlıdere, Elif; Çetin, Aslı
    Öz: Karaciğer ve pankreasın parankiması endoderm, stroması ise mezoderm kaynaklıdır. Her iki organ da özefagus, mide ve duodenumun bir kısmının kaynaklandığı ön bağırsak endoderminden gelişirler. Karaciğer, safra kesesi ve safra kanalları 3. haftanın ortası ile 4. haftanın başında ön bağırsağın kaudal parçasından kaynaklanan diverticulum hepaticum'dan gelişmeye başlarlar. Karaciğer divertikülünün gelişmesinde septum transversumun ve kardiyak mezodermin indükleyici etkileri vardır. Pankreas da önbağırsağın endoderminden kaynaklanır. Pankreasın gelişeceği alanda duodenum endoderminden kaynaklanan dorsal ve ventral pankreas tomurcuklarının daha sonra birleşmesi ile pankreas gelişir. Pankreas gelişiminde yakın komşuluğunda bulunduğu notokorddan ve dorsal aortadan kaynaklanan sinyallerin indükleyici etkileri rol oynar. Bu kısa derlemede karaciğer ve pankreasın morfolojik ve fonksiyonel gelişimleri bu organların prenatal ve postnatal gelişimleri ile ilgili sıçanlardan elde edilen resimler eşliğinde anlatılmıştır.
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    Melatonin is effective in reducing stress-induced organ damage in Wistar albino rats
    (Turkish Journal of Biology, 2014) Eşrefoğlu, Mukaddes; Akıncı, Ayşin; Elbe, Hülya; Taşlıdere, Elif; Taşlıdere, Elif; Çetin, Aslı; Ateş, Burhan
    Abstract: In the present study, we tried to investigate the effects of melatonin, a novel antioxidant and a potent free radical scavenger, in stress-induced cerebral, cerebellar, cardiac, and hepatic oxidative damage using microscopic and biochemical analysis. A total of 32 male Wistar albino rats were divided into control, stress, stress + saline, and stress + melatonin groups. The rats from the stress groups were exposed to high stress conditions of starvation, immobilization, and cold exposure. The rats from the stress + melatonin group received melatonin daily at 20 mg/kg body weight intraperitoneally for 7 days. At the end of the experiment, the brain, cerebellum, heart, and liver were rapidly removed. The main histopathological damage scores (MHDSs) of the stress and stress + saline groups were higher than those of control group for all of the organs. The MHDSs of melatonin-administered group were lower than those of stress and stress + saline groups. The main tissue superoxide dismutase activities of the stress + melatonin group were even higher than those of the control group in the cerebellum and liver, and main tissue catalase activities of the stress + melatonin group were even higher than those of control group in all of the organs. As a conclusion, we found melatonin very effective in reducing stress-induced organ damage by inhibiting lipid peroxidation and supporting the cellular antioxidant defense system.
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    Oral administration of hesperidin a citrus flavonone in rats counteracts the oxidative stress the inflammatory cytokine production and the hepatotoxicity induced by the ingestion of 2 3 7 8 tetrachlorodibenzo p dioxin TCDD
    (Eur.Cytokine Netw, 2013) Bentli, Recep; Çiftçi, Osman; Çetin, Aslı; Ünlü, Merve; Başak, Neşe; Çay, Mahmut
    The objective of the current study was to investigate the protective effects of hesperidin against oxidative stress, altered cytokines levels and histological changes in rats induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were divided randomly into four equal groups (Control, TCDD, hesperidin and TCDD+hesperidin). TCDD and hesperidin were given by gavage, dissolved in corn oil at doses of 2 /kg/week and 50 mg/kg/day respectively. The blood and tissue samples were taken from all rats on the 60th day, to be analyzed for the determination of oxidative stress, histological changes and cytokine levels. The results indicated that hesperidin prevented oxidative damage caused by TCDD via decrease lipid peroxidation and increased antioxidant defense systems. It also reversed the histological damage induced by TCDD. Although, TCDD led to a significant increase in TNF- and IL-1 levels, hesperidin treatment was able to normalize these values in rats. In conclusion, it was shown that TCDD caused adverse effects as regards cytokine levels, histological alterations and oxidative stress in rats. However, hesperidin treatment mitigated these toxic effects. These results suggest that hesperidin could play a protective role against TCDD toxicity.
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    Prenatal and Postnatal Development of the Stomach in Wistar Albino Rats
    (İnönü Üniversitesi Tıp Fakültesi Dergisi, 2014) Çetin, Aslı; Eşrefoğlu, Mukaddes
    Aim: In this study, histological changes in stomach during prenatal and postnatal development were examined. Material and Methods: In this study, 34 female Wistar Albino rats weighing 200-250 g, obtained from Inonu University Experimental Animal Research Lab were used. Stomach samples obtained from prenatal 7,10,14,17, 20 days old fetuses and from postnatal 5,10,15,20 days old newly born and young adult rats were prepared by routine tissue proceeding procedure and examined by light microscopy. Results: In prenatal period, the stomachs of 7,10,14 days old rats were surrounded by stratified columnar or pseudostratified columnar epithelium. Mesenchymal connective tissue surrounded the epithelium. A circular oriented muscle layer was formed in mesenchyme in prenatal 17 days old rats. In prenatal 20 days old rats, extension of the lumen, thickening of the wall, appearance of the foveola and glandlike structures were observed. Epithelium was transformed into simple columnar epithelium in various places. Mucous neck cells in the gland epithelium and outermost serosa layer were identified. On postnatal 5th day, parietal and chief cells could be detected in tubular gastric glands. Myenteric plexus was observed between two muscle layers. On postnatal 10th day, mucus layer was observed on the surface. In subsequent periods, histological properties of stomach were changed and gained adult stomach’s properties. Results: It was investigated in which prenatal and postnatal periods the histological features of the stomach of an adult rat were acquired. The obtained data of this study will guide the other related studies.
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    The protective cardiac effects of myrcene after global cerebral ıschemia reperfusion in C57BL J6 mouse
    (Acta cir bras, 2016) Baykalır, Burcu Gül; Çiftçi, Osman; Çetin, Aslı; Öztanır, Mustafa Namık; Başak, Neşe
    ABSTRACT PURPOSE: To investigate the protective effect of β-myrcene (MYR) on oxidative and histological damage in mice heart tissue caused global cerebral ischemia/reperfusion (IR) in C57BL/J6 mice. METHODS: Animals(n=40) were randomly divided into four groups: (1)control, (2)IR, (3)MYR and (4)MYR+IR. The control group was received 0.1% carboxymethyl cellulose as a vehicle following a medial incision without carotid occlusion. In the IR group, the bilateral carotid arteries were clipped for 15min, and treated with the vehicle intraperitoneally(ip) for 10 days. MYR (200mg/kg) was received dissolved in 0.1%CMC for 10 days. In the MYR+IR group, the IR model was applied exactly as in the IR group, and then they were treated with MYR 10 days. RESULTS: The cerebral IR caused oxidative damage (increase TBARS, decrease antioxidant parameters). Treatment of MYR was increased in GSH,GPx,CAT,SOD activity while TBARS level was decreased. In addition, degenerative changes in I/R group heart tissue were ameliorated by MYR administration. CONCLUSİON: The administration of β-myrcene protects oxidative and histological damage in the heart tissue after global ischemiareperfusion and may be useful safe alternative treatment for cardiac tissue after ischemic stroke.
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    Protective role of Diospyros lotus on cisplatin induced changes in sperm characteristics testicular damage and oxidative stress in rats
    (Andrologia, 2016) Saral, Sinan; Özçelik, E; Saral, O.; Çetin, Aslı; Başak, Neşe; Çiftçi, Osman; Aydın, Muhterem
    The aim of this study was to investigate the protective effect of Diospyros lotus (DL) on cisplatin (CP)-induced testicular damage in male rats. Twenty-eight male rats were randomly divided into four groups: group 1 – control, given isotonic saline solution; group 2 – CP 7 mg kg 1 given intraperitoneally as single dose; group 3 – DL 1000 mg kg 1 per day given orally for 10 days; group 4 – CP and DL given together at the same doses. CP caused a significant increase in thiobarbituric acid-reactive substances (TBARS) level and a significant decrease in superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) levels in rats testis tissues compared to the control group. CP caused a significant increase in lipid peroxidation in testis tissues compared to the control group, whereas DL led to a significant increase in SOD and GSH levels. However, there were no statistically significant changes in GPx and CAT levels. In addition, serum testosterone levels, sperm concentration and sperm motility were significantly decreased, but abnormal sperm rate and histological changes were increased with CP. However, these effects of CP on sperm parameters, histological changes and the tissue weights were eliminated by DL treatment. In conclusion, our study showed that the reproductive toxicity caused by CP may be prevented by DL treatment.