Production of L-DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin gene

Küçük Resim Yok

Tarih

2009

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Given the well-established beneficial effects of Vitreoscilla hemoglobin (VHb) on heterologous organisms, the potential of this protein for the production of L-DOPA and dopamine in two bacteria, Citrobacter freundii and Erwinia herbicola, was investigated. The constructed recombinants bearing the VHb gene (vgb+) had substantially higher levels of cytoplasmic L-DOPA (112 mg/L for C. freundii and 97 mg/L for E. herbicola) than their respective hosts (30.4 and 33.8 mg/L) and the vgb- control strains (35.6 and 35.8 mg/L). Further, the vgb+ recombinants of C. freundii and E. herbicola had 20-fold and about two orders of magnitude higher dopamine levels than their hosts, repectively. The activity of tyrosine phenol-lyase, the enzyme converting L-tyrosine to L-DOPA, was well-correlated to cytoplasmic L-DOPA levels. As cultures aged, higher tyrosine phenol-lyase activity of the vgb+ strains was more apparent. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Açıklama

Anahtar Kelimeler

Dopamine, Erwinia herbicola, L-DOPA, Tyrosine phenol-lyase, Vitreoscilla hemoglobin

Kaynak

Biotechnology Journal

WoS Q Değeri

Scopus Q Değeri

Q1

Cilt

4

Sayı

7

Künye