Production of L-DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin gene

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dc.authorscopusid36465536100
dc.authorscopusid36464917400
dc.authorscopusid58448797900
dc.authorscopusid6507504123
dc.authorscopusid6602871282
dc.contributor.authorKurt A.G.
dc.contributor.authorAytan E.
dc.contributor.authorOzer U.
dc.contributor.authorAtes B.
dc.contributor.authorGeckil H.
dc.date.accessioned2024-08-04T20:02:36Z
dc.date.available2024-08-04T20:02:36Z
dc.date.issued2009
dc.departmentİnönü Üniversitesien_US
dc.description.abstractGiven the well-established beneficial effects of Vitreoscilla hemoglobin (VHb) on heterologous organisms, the potential of this protein for the production of L-DOPA and dopamine in two bacteria, Citrobacter freundii and Erwinia herbicola, was investigated. The constructed recombinants bearing the VHb gene (vgb+) had substantially higher levels of cytoplasmic L-DOPA (112 mg/L for C. freundii and 97 mg/L for E. herbicola) than their respective hosts (30.4 and 33.8 mg/L) and the vgb- control strains (35.6 and 35.8 mg/L). Further, the vgb+ recombinants of C. freundii and E. herbicola had 20-fold and about two orders of magnitude higher dopamine levels than their hosts, repectively. The activity of tyrosine phenol-lyase, the enzyme converting L-tyrosine to L-DOPA, was well-correlated to cytoplasmic L-DOPA levels. As cultures aged, higher tyrosine phenol-lyase activity of the vgb+ strains was more apparent. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.identifier.doi10.1002/biot.200900130
dc.identifier.endpage1088en_US
dc.identifier.issn1860-6768
dc.identifier.issue7en_US
dc.identifier.pmid19585534en_US
dc.identifier.scopus2-s2.0-70350241260en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1077en_US
dc.identifier.urihttps://doi.org/10.1002/biot.200900130
dc.identifier.urihttps://hdl.handle.net/11616/91823
dc.identifier.volume4en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDopamineen_US
dc.subjectErwinia herbicolaen_US
dc.subjectL-DOPAen_US
dc.subjectTyrosine phenol-lyaseen_US
dc.subjectVitreoscilla hemoglobinen_US
dc.titleProduction of L-DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin geneen_US
dc.typeArticleen_US

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