Comparison of Epigenetic Changes of Endoplasmic Reticulum Stress and Autophagic Pathways in Various Cancer Cell Lines

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dc.contributor.authorÖzgen, Nazmi
dc.contributor.authorYüksel, Şengül
dc.date.accessioned2026-04-04T13:19:06Z
dc.date.available2026-04-04T13:19:06Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractDisruption of the balance between processed protein load and protein folding capacity in the endoplasmic reticulum (ER), where post-translational modifications occur, results in the occurrence of adaptive ER stress, leading to the expectation of active autophagy. The relationship between ER stress and autophagic dysfunctions with cancer remains uncertain. The aim of this study is to investigate methylation changes in key genes in various cancer cells within these pathways. In this study, methylation changes in some major genes involved in ER stress and autophagic pathways were investigated in various cancer cells (HepG2, MCF-7) and healthy cells (MCF-10A) using the RT-PCR technique. Our study found that in MCF7 cells, the DNA methylation levels of ER stress genes MAP3K5, ATF6, and MAOA significantly decreased, while they increased in the XBP1 and EIF2AK3 genes. In HEPG2 cells, hypomethylation was detected in MAP3K5, EIF2AK3, and MAOA genes. Only GBA gene showed significant hypomethylation in both cells among autophagy pathway genes. With these results, it can be speculated that the epigenetic activities of most protein genes in ER stress and autophagic pathways are altered in cancer cells, thus accelerating the transformation process of cells. © 2025, Inonu University. All rights reserved.
dc.identifier.doi10.33715/inonusaglik.1544914
dc.identifier.endpage180
dc.identifier.issn2147-7892
dc.identifier.issue1
dc.identifier.scopus2-s2.0-105000955375
dc.identifier.scopusqualityQ4
dc.identifier.startpage169
dc.identifier.trdizinid1301702
dc.identifier.urihttps://doi.org/10.33715/inonusaglik.1544914
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1301702
dc.identifier.urihttps://hdl.handle.net/11616/108162
dc.identifier.volume13
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isotr
dc.publisherInonu University
dc.relation.ispartofJournal of Inonu University Vocational School of Health Services
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250329
dc.subjectAutophagy
dc.subjectCancer
dc.subjectDNA Methylation
dc.subjectER stress
dc.subjectHepG2
dc.subjectMCF-10A
dc.subjectMCF-7
dc.titleComparison of Epigenetic Changes of Endoplasmic Reticulum Stress and Autophagic Pathways in Various Cancer Cell Lines
dc.title.alternativeKANSER HÜCRELERİNDE ER STRESİ VE OTOFAJİK YOLAKLARDAKİ GENLERİN EPİGENETİK DEĞİŞİKLİKLERİNİN ARAŞTIRILMASI]
dc.typeArticle

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