Design of an electrochemical sensor based on 3-thiophene methanol and 3-methylthiophene supported molecularly imprinted polymer for the sensitive and selective detection of levothyroxine
Küçük Resim Yok
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Levothyroxine (L-T4) is the most frequently prescribed drug to treat thyroid hormone deficiency. Herein, a levothyroxine-specific sensor was designed using the molecular imprinting method to increase the selectivity of the target molecule on the electrode surface and prevent interference effects. 3-thiophene methanol (3-TMeOH) and 3-methyl thiophene (3-MT) monomers were electro-polymerized as molecularly imprinted polymer (MIP) on the surface of a glassy carbon electrode (GCE) in the presence of L-T4. Gaps were created on the surface of the designed electrode to fit the target molecule by removing L-T4 molecules. This way, L-T4-specific selective structures were formed on the sensors' surface. The results showed that under optimized conditions, the fabricated modified sensor (3-TMeOH/3-MT@MIP/GCE) exhibited a linear response in the concentration range of 1.0-5.7 mu M with limit of determination, limit of quantitation, and sensitivity values of 1.83 nM, 5.50 nM, and 0.0023 A.L.mmol- 1.cm- 2, respectively. The analytical method validation process of the voltammetric determination method using the sensor selective to L-T4 was completed following the method's intended use. The designed modified sensor exhibited high sensitivity, repeatability, excellent stability, selectivity, and high precision and accuracy.
Açıklama
Anahtar Kelimeler
Electroanalytical sensor, Hypothyroidism, Levothyroxine, Molecularly imprinted polymer, Voltammetry
Kaynak
Microchemical Journal
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
218
