The effects of phenoxybenzamine on tyrosine hydroxylase (TH) and mRNA level in adrenal medulla of sprague dawley rats
Yükleniyor...
Dosyalar
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Journal of Medical Sciences
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Abstract: The effects of anti hipertensive phenoxybenzamine were investigated on tyrosine hydroxylase (TH) enzyme activity and TH mRNA levels. In the present study 5 months male sprague dawley (SD) rats were used. Phenoxybenzamine was injected i.p as 20 mg/kg which prepared in the 0.9 % NaCI and 5% ethanole. TH activity was measured by detecting of formation of 3H2O as a formation of dopa from 3H-tyrosine by radioisotope technique total adrenal RNA was isolated and hybridized wit 32P labeled cDNA. TH mRNA was assayed by densitometric scanning of the autoradiograms using a densitometer. TH activity and TH mRNA levels were found to be significantly increased by the effcet of phenoxybenzamine (P<0.01).
Açıklama
Yıl: 1998Cilt: 28Sayı: 1ISSN: 1300-0144 / 1303-6165Sayfa Aralığı: 35 - 39Metin Dili:İngilizce
Anahtar Kelimeler
Kaynak
Turkish Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
28
Sayı
1
Künye
YÜREKLİ M (1998). The effects of phenoxybenzamine on tyrosine hydroxylase (TH) and mRNA level in adrenal medulla of sprague dawley rats. Turkish Journal of Medical Sciences, 28(1), 35 - 39.
