Synthesis and characterization of biodegradable pHEMA-starch composites for immobilization of L-asparaginase
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this paper, poly (2-hydroxyethyl methacrylate) (pHEMA) and poly (2-hydroxyethyl methacrylate)-starch (pHEMA-S) composites were prepared from 2-hydroxyethyl methacrylate and different ratio starch. The pHEMA-S showed high thermal stability and biodegradability. The biodegradability of pHEMA-S composites was in the range of 14-22 % for 4 weeks depending on the starch ratio of pHEMA-S. Then, L-ASNase was immobilized onto pHEMA-S composites. The immobilized L-ASNase was investigated with optimum temperature, pH and kinetic parameters. Immobilization improved the pH stability and thermal stability of the enzyme. The K-m values were 1.58 and 0.56 mM for free and immobilized enzyme, respectively.
Açıklama
Anahtar Kelimeler
L-asparaginase, pHEMA, pHEMA-starch composites, Immobilization
Kaynak
Polymer Bulletin
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
73
Sayı
7
