First report of Candidatus Phytoplasma solani on a new host marigold (Tagetes erecta L.)

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dc.authoridALP, Sevket/0000-0002-9552-4848
dc.authorwosidSipahioglu, Hikmet Murat/AAA-6085-2020
dc.authorwosidUsta, Mustafa/CAG-7521-2022
dc.contributor.authorAlp, Sevket
dc.contributor.authorUsta, Mustafa
dc.contributor.authorSipahioglu, Hikmet Murat
dc.contributor.authorGuller, Abdullah
dc.date.accessioned2024-08-04T20:09:49Z
dc.date.available2024-08-04T20:09:49Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractMarigold (Tagetes erecta L.) plants, also called Mexican or Aztec marigold, with symptoms of shoot proliferation, dwarfing, and reddening were observed in ornamental gardens of Van Province (Turkey). Five plants, two of them showing reddening and three symptomless plants, were sampled at the end of September 2014. Genomic DNA isolated from symptomatic and nonsymptomatic plant leaves was used to amplify 16S rDNA fragments by nested polymerase chain reaction (PCR). Of the 5 marigold samples tested by PCR, only the two showing reddening symptoms yielded the expected 1.2-kb DNA fragments. Amplified PCR fragments were cloned into a plasmid vector and transformed into competent Escherichia coli strain JM 109. Recombinant plasmid DNA was isolated and sequenced bidirectionally. The provided sequences were 1244 bp and 1245 bp in length and were designated as isolate 1 and isolate 2, respectively. BLAST analysis of the 16S rDNA sequence and virtual restriction fragment length polymorphism (RFLP) analysis confirmed the presence of the phytoplasma Candidatus Phytoplasma solani. The in silico virtual RFLP pattern of isolate 1, based on the 16S rDNA F2n/R2 fragment, was identical (similarity coefficient 1.00) to the reference pattern of 16Sr group XII, subgroup A (GenBank accession no.: AF248959). Isolate 1 was identified as a member of 16SrXII-A. Based on the same analyses, isolate 2 showed molecular characteristics different from reference patterns of all previously established 16Sr groups and subgroups. The most similar was the reference pattern of 16Sr group XII, subgroup A (GenBank accession no.: AF248959), with a similarity coefficient of 0.97. This is the first report of naturally occurring Ca. P. solani affecting T. erecta, which shows that this plant species is an alternate host of the agent.en_US
dc.identifier.doi10.3906/tar-1506-58
dc.identifier.endpage318en_US
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84969170264en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage311en_US
dc.identifier.trdizinid214524en_US
dc.identifier.urihttps://doi.org/10.3906/tar-1506-58
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/214524
dc.identifier.urihttps://hdl.handle.net/11616/92457
dc.identifier.volume40en_US
dc.identifier.wosWOS:000378090100002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Agriculture and Forestryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCandidatus Phytoplasma solanien_US
dc.subjectTagetes erectaen_US
dc.subjectnested PCRen_US
dc.subjectmolecular cloningen_US
dc.titleFirst report of Candidatus Phytoplasma solani on a new host marigold (Tagetes erecta L.)en_US
dc.typeArticleen_US

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