Sıçanlarda karbon tetraklorür ile indüklenen kardiyak hasar üzerine melatonin ve quercetin'in tedavi edici etkileri
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Tarih
2016
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz: Amaç: Karbontetraklorür (CCl4) potent, lipidde çözülebilir toksik bir ajandır ve çoğu dokuda peroksidatif değişikliklere neden olur. Bu çalışmada, CCl4 ile indüklenen kalp hasarında melatonin ve quercetinin antioksidatif etkileri histolojik ve biyokimyasal yöntemler yoluyla incelendi. Gereç ve Yöntem: 35 adet sıçan eşit olarak 5 gruba ayrıldı. Kontrol, zeytinyağı, CCl4, CCl4+Melatonin ve CCl4+Quercetin. Deney sonunda kalp dokuları rutin histolojik ve biyokimyasal prosedürlerden geçirildi. Bulgular: Kontrol ve zeytinyağı gruplarının histolojik görüntüsü normaldi. CCl4 grubunda ise; kardiyositlerde vakuolizasyon ve nekroz, vasküler konjesyon, hemoraji, infiltrasyon ve fibrozis gibi histopatolojik bulgular gözlendi. Kontrol ve zeytinyağı gruplarıyla karşılaştırıldığında, CCl4 grubunun histopatolojik skorunda anlamlı bir artış tespit edildi. Diğer yandan, histopatolojik değişiklikler CCl4+Melatonin ve CCl4+Quercetin gruplarında önemli ölçüde geriledi. CCl4 grubunun doku MDA düzeyleri kontrol ve zeytinyağı grupları ile karşılaştırıldığında anlamlı derecede artmıştı. Tedavi gruplarının MDA düzeyleri CCl4 grubundan anlamlı derecede düşüktü. Kontrol ve zeytinyağı grupları ile karşılaştırıldığında doku GSH düzeyleri ve SOD aktivitesi anlamlı derecede azalmıştı. Melatonin ve quercetin uygulamaları GSH düzeylerinde ve SOD aktivitesinde anlamlı artışlarla sonuçlandı. Sonuç: CCl4 toksisitesinde oluşan kalp hasarının, antioksidatif özellikleri vasıtasıyla melatonin ve quercetin uygulamaları ile önlenebileceğini düşünmekteyiz.
Abstract: Aim: Carbon tetrachloride (CCl4) is a potent, lipid-soluble toxic agent and produces peroxidative degeneration in many tissues. In this study, antioxidative effects of melatonin and quercetin were evaluated by histological and biochemical methods in CCl4-induced cardiac damage. Materials and Method: 35 rats were divided into equally 5 groups: control, olive oil, CCl4, CCl4+Melatonin, and CCl4+Quercetin. At the end of the study hearts tissues were processed by routin histological and biochemical procedures. Results: The histological appearance of control and olive oil groups were normal. However, histopathological alterations such as vacuolization and necrosis of cardiocytes, vascular congestion, hemorrhage, infiltration and fibrosis were observed in the CCl4 group. Significant increase in histopathological scoring was detected in CCl group when compared with the control and olive oil groups. Furthermore, histopathological changes were markedly reduced in CCl4+Melatonin, and CCl4+Quercetin. The tissue MDA levels of CCl4 group were significantly increased in comparison with the control and olive oil groups. In treatment groups, MDA levels were significantly lower than that of the CCl4 group. The tissue GSH levels and SOD activities were significantly decreased when compared with the control and olive oil groups. Melatonin and quercetin administrations resulted in significant increases in GSH levels and SOD activities. Conclusion: We concluded that cardiac damage induced by CCl4 toxicity is prevented by melatonin and quercetin administrations via antioxidative properties.
Abstract: Aim: Carbon tetrachloride (CCl4) is a potent, lipid-soluble toxic agent and produces peroxidative degeneration in many tissues. In this study, antioxidative effects of melatonin and quercetin were evaluated by histological and biochemical methods in CCl4-induced cardiac damage. Materials and Method: 35 rats were divided into equally 5 groups: control, olive oil, CCl4, CCl4+Melatonin, and CCl4+Quercetin. At the end of the study hearts tissues were processed by routin histological and biochemical procedures. Results: The histological appearance of control and olive oil groups were normal. However, histopathological alterations such as vacuolization and necrosis of cardiocytes, vascular congestion, hemorrhage, infiltration and fibrosis were observed in the CCl4 group. Significant increase in histopathological scoring was detected in CCl group when compared with the control and olive oil groups. Furthermore, histopathological changes were markedly reduced in CCl4+Melatonin, and CCl4+Quercetin. The tissue MDA levels of CCl4 group were significantly increased in comparison with the control and olive oil groups. In treatment groups, MDA levels were significantly lower than that of the CCl4 group. The tissue GSH levels and SOD activities were significantly decreased when compared with the control and olive oil groups. Melatonin and quercetin administrations resulted in significant increases in GSH levels and SOD activities. Conclusion: We concluded that cardiac damage induced by CCl4 toxicity is prevented by melatonin and quercetin administrations via antioxidative properties.
Açıklama
Anahtar Kelimeler
Kaynak
Turgut Özal Tıp Merkezi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
23
Sayı
2
Künye
ELBE H,EŞREFOĞLU M,TASLİDERE A,TAŞLIDERE E,ATEŞ B (2016). Sıçanlarda karbon tetraklorür ile indüklenen kardiyak hasar üzerine melatonin ve quercetin'in tedavi edici etkileri. İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi, 23(2), 171 - 176.
