Effects of low-intensity pulsed ultrasound on bone formation after the expansion of the inter-premaxillary suture in rats: a histologic and immunohistochemical study
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sciendo
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: Orthodontic maxillary expansion is a commonly-performed treatment approach to correct transverse maxillary deficiencies by separating the mid-palatal suture. To obtain a successful treatment result and prevent relapse, the expanded maxillary sutures require maintenance by means of qualified bone reorganisation. Aim: To assess the effects of low-intensity pulsed ultrasound (LIPUS) on sutural bone formation after the expansion of the interpremaxillary suture in rats. Methods: Sixteen male Wistar rats, 6 to 8-week old, were used. The expansion appliance comprised a helical spring fabricated from 0.014 inch stainless steel wire (Dentaurum, Ispringen, Germany). The rats were divided into two equal groups and randomly assigned to the LIPUS treatment group or a sham-operation group. LIPUS was delivered via a 2.5 cm diameter ultrasound transducer (Exogen, Smith and Nephews, Inc., Memphis, TN, USA) for 20 minutes per day during 7 days of postexpansion retention. Following retention, the rats' maxillae were surgically removed and histologic and immunohistochemical specimens were prepared and examined. Results: The number of osteoblasts and blood vessel dimensions in the ultrasound group increased but was not significant, compared with the control group. A statistically significant difference in osteocalcin, VEGF and TGF-beta immunoreactivities (p < 0.01) was found in the area of the mineralising tissue. Only VEGF immunoreactivity was significant between two groups (p < 0.01) in the fibrous tissue area. Conclusions: The area of mineralising tissue in the LIPUS-applied group expressed activity markers for osteocalcin, VEGF and TGF-beta compared with a surrounding area of fibrous tissue. Cellular activation in the LIPUS group was greater than that of controls. Therefore, LIPUS may be accepted as a useful approach to enhance sutural bone formation. (Aust Orthod J 2014; 30: 176-183)
Açıklama
Anahtar Kelimeler
Orthopedically Expanded Suture, Midpalatal Suture, Maxillary Expansion, Induced Root, Stimulation, Laser, Resorption, Repair
Kaynak
Australasian Orthodontic Journal
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
30
Sayı
2
