Effects of low-intensity pulsed ultrasound on bone formation after the expansion of the inter-premaxillary suture in rats: a histologic and immunohistochemical study

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dc.authoridKozacioglu, Sumeyye/0000-0002-5842-4063
dc.authorwosidKozacioglu, Sumeyye/GPT-5564-2022
dc.contributor.authorToy, Ebubekir
dc.contributor.authorOzturk, Firat
dc.contributor.authorAltindis, Sedat
dc.contributor.authorKozacioglu, Sumeyye
dc.contributor.authorToy, Hatice
dc.date.accessioned2024-08-04T20:40:03Z
dc.date.available2024-08-04T20:40:03Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Orthodontic maxillary expansion is a commonly-performed treatment approach to correct transverse maxillary deficiencies by separating the mid-palatal suture. To obtain a successful treatment result and prevent relapse, the expanded maxillary sutures require maintenance by means of qualified bone reorganisation. Aim: To assess the effects of low-intensity pulsed ultrasound (LIPUS) on sutural bone formation after the expansion of the interpremaxillary suture in rats. Methods: Sixteen male Wistar rats, 6 to 8-week old, were used. The expansion appliance comprised a helical spring fabricated from 0.014 inch stainless steel wire (Dentaurum, Ispringen, Germany). The rats were divided into two equal groups and randomly assigned to the LIPUS treatment group or a sham-operation group. LIPUS was delivered via a 2.5 cm diameter ultrasound transducer (Exogen, Smith and Nephews, Inc., Memphis, TN, USA) for 20 minutes per day during 7 days of postexpansion retention. Following retention, the rats' maxillae were surgically removed and histologic and immunohistochemical specimens were prepared and examined. Results: The number of osteoblasts and blood vessel dimensions in the ultrasound group increased but was not significant, compared with the control group. A statistically significant difference in osteocalcin, VEGF and TGF-beta immunoreactivities (p < 0.01) was found in the area of the mineralising tissue. Only VEGF immunoreactivity was significant between two groups (p < 0.01) in the fibrous tissue area. Conclusions: The area of mineralising tissue in the LIPUS-applied group expressed activity markers for osteocalcin, VEGF and TGF-beta compared with a surrounding area of fibrous tissue. Cellular activation in the LIPUS group was greater than that of controls. Therefore, LIPUS may be accepted as a useful approach to enhance sutural bone formation. (Aust Orthod J 2014; 30: 176-183)en_US
dc.description.sponsorshipInoenue University Research Fund, Malatya, Turkey [2011/191]en_US
dc.description.sponsorshipThe financial support for this study was granted from Inoenue University Research Fund, Malatya, Turkey (No: 2011/191). We thank Dr. Harika G. G. Bag, Assistant Professor in the Department of Biostatistics, Inoenue University, for her skilful statistical assistance.en_US
dc.identifier.doi10.2478/aoj-2014-0015
dc.identifier.endpage183en_US
dc.identifier.issn2207-7472
dc.identifier.issn2207-7480
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84922393216en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage176en_US
dc.identifier.urihttps://doi.org/10.2478/aoj-2014-0015
dc.identifier.urihttps://hdl.handle.net/11616/96678
dc.identifier.volume30en_US
dc.identifier.wosWOS:001046439700007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSciendoen_US
dc.relation.ispartofAustralasian Orthodontic Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOrthopedically Expanded Sutureen_US
dc.subjectMidpalatal Sutureen_US
dc.subjectMaxillary Expansionen_US
dc.subjectInduced Rooten_US
dc.subjectStimulationen_US
dc.subjectLaseren_US
dc.subjectResorptionen_US
dc.subjectRepairen_US
dc.titleEffects of low-intensity pulsed ultrasound on bone formation after the expansion of the inter-premaxillary suture in rats: a histologic and immunohistochemical studyen_US
dc.typeArticleen_US

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