The protective effects of epigallocatechin-3-gallate (EGCG) on hydrogen peroxide-induced oxidative damages in Saccharomyces cerevisiae
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mattioli 1885
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Epigallocatechin gallate (EGCG), one of the green tea ingredients, is a non-toxic catechin derivative and is an effective polyphenol in preventing tissue damage, cancer formation and increasing metabolic rate. Strong antioxidant property EGCG having anti-inflammatory, antioxidant, antihypertensive, antimicrobial, antiviral, antifungal, antitumor and various organs as well as neuroprotective effect of many convenience protection against ischemia-reperfusion injury. In this study, four groups were formed to investigate whether Epigallocatechin-3-gallate (EGCG) has a protective role against the damage caused by hydrogen peroxide (H2O2) in Saccharomyces cerevisiae. Groups: (i) Control Group; Yeast cultivated group only; (ii) EGCG Group: EGCG administered group (10 %); (iii) H2O2 Group: Group given H2O2 (15 mM); (iv) EGCG + H2O2 (15 mM) Group: Group given EGCG (10 %) + H2O2 (15 mM). S. cerevisiae cultures were developed at 30 degrees C for 1, 3, 5 and 24 hours (h). Cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) levels were determined by spectrophotometer. Total protein changes were detected by SDS-PAGE electrophoresis and calculated by Bradford method. According to the results obtained, Cell growth (1, 3, 5 and 24 h), total protein synthesis and GSH levels (24 h) increased in EGCG groups, while MDA level decreased (24 h) when compared with H2O2 group. As a result, EGCG has been shown to have an effect that promotes cell growth and total protein synthesis as well as reducing oxidative damage in S. cerevisiae culture.
Açıklama
Anahtar Kelimeler
EGCG, H2O2, oxidative damage, protein, Saccharomyces cerevisiae, SDS-PAGE
Kaynak
Progress in Nutrition
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
23
Sayı
2
