The protective effects of epigallocatechin-3-gallate (EGCG) on hydrogen peroxide-induced oxidative damages in Saccharomyces cerevisiae

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dc.authoridBEYAZ, SEDA/0000-0003-0436-8112
dc.authoridBeyaz, Seda/0000-0003-0436-8112
dc.authoridGök, Özlem/0000-0001-8521-6369
dc.authoridCan, Muhammed Ismail/0000-0002-0118-2278
dc.authorwosidcan, mi/KDN-4165-2024
dc.authorwosidBEYAZ, SEDA/ABI-5835-2020
dc.authorwosidBeyaz, Seda/ABD-1252-2021
dc.authorwosidGök, Özlem/AFV-8223-2022
dc.authorwosidCan, Muhammed İsmail/ABG-8646-2020
dc.authorwosidAslan, Abdullah/C-9523-2016
dc.contributor.authorBeyaz, Seda
dc.contributor.authorGok, Ozlem
dc.contributor.authorCan, Muhammed Ismail
dc.contributor.authorAslan, Abdullah
dc.date.accessioned2024-08-04T20:50:24Z
dc.date.available2024-08-04T20:50:24Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractEpigallocatechin gallate (EGCG), one of the green tea ingredients, is a non-toxic catechin derivative and is an effective polyphenol in preventing tissue damage, cancer formation and increasing metabolic rate. Strong antioxidant property EGCG having anti-inflammatory, antioxidant, antihypertensive, antimicrobial, antiviral, antifungal, antitumor and various organs as well as neuroprotective effect of many convenience protection against ischemia-reperfusion injury. In this study, four groups were formed to investigate whether Epigallocatechin-3-gallate (EGCG) has a protective role against the damage caused by hydrogen peroxide (H2O2) in Saccharomyces cerevisiae. Groups: (i) Control Group; Yeast cultivated group only; (ii) EGCG Group: EGCG administered group (10 %); (iii) H2O2 Group: Group given H2O2 (15 mM); (iv) EGCG + H2O2 (15 mM) Group: Group given EGCG (10 %) + H2O2 (15 mM). S. cerevisiae cultures were developed at 30 degrees C for 1, 3, 5 and 24 hours (h). Cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) levels were determined by spectrophotometer. Total protein changes were detected by SDS-PAGE electrophoresis and calculated by Bradford method. According to the results obtained, Cell growth (1, 3, 5 and 24 h), total protein synthesis and GSH levels (24 h) increased in EGCG groups, while MDA level decreased (24 h) when compared with H2O2 group. As a result, EGCG has been shown to have an effect that promotes cell growth and total protein synthesis as well as reducing oxidative damage in S. cerevisiae culture.en_US
dc.identifier.doi10.23751/pn.v23i2.11039
dc.identifier.issn1129-8723
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85109435458en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.23751/pn.v23i2.11039
dc.identifier.urihttps://hdl.handle.net/11616/100033
dc.identifier.volume23en_US
dc.identifier.wosWOS:000669548500032en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMattioli 1885en_US
dc.relation.ispartofProgress in Nutritionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEGCGen_US
dc.subjectH2O2en_US
dc.subjectoxidative damageen_US
dc.subjectproteinen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectSDS-PAGEen_US
dc.titleThe protective effects of epigallocatechin-3-gallate (EGCG) on hydrogen peroxide-induced oxidative damages in Saccharomyces cerevisiaeen_US
dc.typeArticleen_US

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