Antiviral and Antifungal Activity of Biologically Active Recombinant Bouganin Protein from Bougainvillea spectabilis Willd

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dc.authorwosidUsta, Mustafa/CAG-7521-2022
dc.contributor.authorGuller, Abdullah
dc.contributor.authorSiphioglu, Hikmet Murat
dc.contributor.authorUsta, Mustafa
dc.contributor.authorDemirer Durak, Emre
dc.date.accessioned2024-08-04T20:09:56Z
dc.date.available2024-08-04T20:09:56Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBouganin antiviral protein (BAP) gene. one of the ribosome inactivating proteins, isolated from Bougainvillea speclabilis Willd. was cloned, expressed and the antiviral and antifungal activities were investigated. The full-length bouganin antiviral protein gene was amplified by reverse transcription-PCR using mRNA as template extracted from mature leaves. The coding region of bouganin gene was cloned into prokaryotic expression vector pETDuet-1 after amplification with end to end gene specific primers. The recombinant plasmid was transformed into Escherichia coli cells BL21(DE3)pLysS and the expression of BAP gene was induced by isopropyl beta-D thiogalactopyranoside (IPTG). Bouganin antiviral protein having a molecular mass of 28 kDa has been isolated from transformed bacterial colonies. Antiviral activity of bouganin was assayed against Zucchini yellow mosaic virus (ZYMV) by a mechanical inoculation test. The antifungal activity of purified recombinant protein was tested against pathogenic and non-pathogenic Rhizocionia solani. Trichoderma harzianum, and Fusarium arysporurn fungi using disc diffusion method. The increased amount of antiviral protein reduced the disease severity caused by ZYMV. The bouganin antiviral protein was inhibited the growth of R. solcati by 30.7% and of T harzianum by 20% after 72 h compared to control. No growth inhibition was observed for F oayporurn. All plants including controls treated with in vitro expressed BAP protein exhibited severe growth reduction compared with negative control (not treated) plants.en_US
dc.description.sponsorshipResearch Fund of University of Van Yuzuncu Yil (YYU-BAP) [2013-FBE-D012]en_US
dc.description.sponsorshipThis research was financially supported by the Research Fund of University of Van Yuzuncu Yil (YYU-BAP, Project no: 2013-FBE-D012).en_US
dc.identifier.doi10.15832/ankutbd.446442
dc.identifier.endpage237en_US
dc.identifier.issn2148-9297
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85048283647en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage227en_US
dc.identifier.trdizinid286276en_US
dc.identifier.urihttps://doi.org/10.15832/ankutbd.446442
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/286276
dc.identifier.urihttps://hdl.handle.net/11616/92498
dc.identifier.volume24en_US
dc.identifier.wosWOS:000440821100009en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherAnkara Univ, Fac Agren_US
dc.relation.ispartofJournal of Agricultural Sciences-Tarim Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBougainvillea spectabilisen_US
dc.subjectAntimicrobial proteinen_US
dc.subjectBAP geneen_US
dc.subjectExpressionen_US
dc.subjectAntiviral and antifungal activitiesen_US
dc.titleAntiviral and Antifungal Activity of Biologically Active Recombinant Bouganin Protein from Bougainvillea spectabilis Willden_US
dc.typeArticleen_US

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